Histone H4 [ac Lys8] Antibody

Novus Biologicals | Catalog # NBP2-42863

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Histone H4 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys8 of human Histone H4. The exact sequence is proprietary.

Modification

ac Lys8

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H4 [ac Lys8] Antibody

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863]

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863]

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.
Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.
Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863]

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863]

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse duodenum. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.
Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863]

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863]

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse kidney. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.
Dot Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody 1:5000 dilution. A: Peptide samples of H4K8 un B: Peptide samples of H4K8ac C: Peptide samples of H4K16un D: Peptide samples of H4K16ac.
Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863]

Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863]

Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa whole cell lysate/extract:
A. 30 ug whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18 hrs)
B. Control with 2.5 ug of pre-immune rabbit IgG
C. Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 ug of Histone H4 (acetyl K9) antibody. 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9).
EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Histone H4 [ac Lys8] Antibody

Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K8ac (acetyl Lys8) antibody (NBP2-42863) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Histone H4 [ac Lys8] Antibody

Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys8] Antibody [NBP2-42863] -

Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody (NBP2-42863) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for Histone H4 [ac Lys8] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:5000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone H4

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4, H4K8ac

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents for Histone H4 [ac Lys8] Antibody

Certificate of Analysis

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Product Specific Notices for Histone H4 [ac Lys8] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Epigenetics
Notch Pathway

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail