HLA DMA Antibody (5C9NB) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-44301

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5C9NB

Format

Azide and BSA Free
View all HLA DMA Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human HLA DMA protein (between amino acids 10-300) [Entrez NP_006111]

Localization

Membranes of Late Endosomes / Lysosomes

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HLA DMA Antibody (5C9NB) - Azide and BSA Free

Western Blot: HLA DMA Antibody (5C9NB)Azide Free [NBP2-44301]

Western Blot: HLA DMA Antibody (5C9NB)Azide Free [NBP2-44301]

Western Blot: HLA DMA Antibody (5C9NB) - Azide Free [NBP2-44301] - Western blot analysis of HLA DMA (5C9NB) in A. partial recombinant human HLA DMA protein B. Ramos lysate and C. Raji lysate. Image using the standard format of this product.
Immunocytochemistry/ Immunofluorescence: HLA DMA Antibody (5C9NB) - Azide Free [NBP2-44301]

Immunocytochemistry/ Immunofluorescence: HLA DMA Antibody (5C9NB) - Azide Free [NBP2-44301]

Immunocytochemistry/Immunofluorescence: HLA DMA Antibody (5C9NB) - Azide Free [NBP2-44301] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HLA DMA (5C9NB) [NBP2-44301-azide free] at a 1:40 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: HLA DMA Antibody (5C9NB) - Azide Free [NBP2-44301]

Immunohistochemistry: HLA DMA Antibody (5C9NB) - Azide Free [NBP2-44301]

Immunohistochemistry: HLA DMA Antibody (5C9NB) - Azide Free [NBP2-44301] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone 5C9NB). The antibody generated a diffused to punctate staining in cellular membranes and cytoplasm, especially in the perinuclear region representing late endosomal localization of major histocompatibility complex, class II, DM alpha/HLA DMA protein. Image using the standard format of this product.
Flow (Cell Surface): HLA DMA Antibody (5C9NB) - Azide Free [NBP2-44301]

Flow (Cell Surface): HLA DMA Antibody (5C9NB) - Azide Free [NBP2-44301]

Flow (Cell Surface): HLA DMA Antibody (5C9NB) - Azide Free [NBP2-44301] - Human PBMC cells were stained (surface) with HLA DMA (5C9NB) antibody (red) or isotype control (mouse IgG1; green). Blue line represents cells alone. Positive staining was observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells were gated (FL-2) for analysis.

Applications for HLA DMA Antibody (5C9NB) - Azide and BSA Free

Application
Recommended Usage

Flow (Cell Surface)

2.5 ug / million cells

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

2 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HLA DMA

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.

Alternate Names

class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM

Gene Symbol

HLA-DMA

Additional HLA DMA Products

Product Documents for HLA DMA Antibody (5C9NB) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for HLA DMA Antibody (5C9NB) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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