HMGB2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38694

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HMGB2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AKGKSEAGKKGPGRPTGSKKKNEP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HMGB2 Antibody - BSA Free

Western Blot: HMGB2 Antibody [NBP2-38694]

Western Blot: HMGB2 Antibody [NBP2-38694]

Western Blot: HMGB2 Antibody [NBP2-38694] - Analysis in human cell lines U2OS and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Immunocytochemistry/ Immunofluorescence: HMGB2 Antibody [NBP2-38694]

Immunocytochemistry/ Immunofluorescence: HMGB2 Antibody [NBP2-38694]

Immunocytochemistry/Immunofluorescence: HMGB2 Antibody [NBP2-38694] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Analysis in human bone marrow and skeletal muscle tissues using NBP2-38694 antibody. Corresponding HMGB2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694]

Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human colon shows strong nuclear positivity in glandular cells.

Applications for HMGB2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB2

HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

Alternate Names

High mobility group protein 2, high mobility group protein B2, high-mobility group box 2, HMG-2, HMG2high-mobility group (nonhistone chromosomal) protein 2

Gene Symbol

HMGB2

UniProt

P26583 (Human)

Additional HMGB2 Products

Product Documents for HMGB2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HMGB2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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