HNF-3 alpha/FoxA1 Antibody - Azide Free

Novus Biologicals | Catalog # NBP1-30877

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all HNF-3 alpha/FoxA1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HNF-3 alpha/FoxA1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (100%), Monopterus albus (100%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HNF-3 alpha/FoxA1 Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP1-30877]

Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP1-30877]

Immunocytochemistry/Immunofluorescence: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Human cervical carcinoma. FOXA1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Chromatin Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877]

Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Human breast carcinoma. FOXA1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877]

Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877]

Immunoprecipitation: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 ug whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of FOXA1 by 2.5 ug of FOXA1 antibody 10% SDS-PAGE The immunoprecipitated FOXA1 protein was detected by FOXA1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
HNF-3 alpha/FoxA1 Antibody

Western Blot: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] -

Western Blot: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
HNF-3 alpha/FoxA1 Antibody - Azide Free

Western Blot: HNF-3 alpha/FoxA1 Antibody [NBP1-30877] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HNF-3 alpha/FoxA1 antibody (NBP1-30877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for HNF-3 alpha/FoxA1 Antibody - Azide Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HNF-3 alpha/FoxA1

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Long Name

Hepatocyte Nuclear Factor-3 alpha/Forkhead Box Protein A1

Alternate Names

FoxA1, HNF3 alpha, TCF3A

Gene Symbol

FOXA1

Additional HNF-3 alpha/FoxA1 Products

Product Documents for HNF-3 alpha/FoxA1 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HNF-3 alpha/FoxA1 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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