hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25508

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1706 expressed in HEK293

Format

Azide and BSA Free
View all hnRNP A2B1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

Western Blot: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] -

Western Blot: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody [HL1706] (NBP3-25508) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

Western Blot: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] -

Western Blot: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody [HL1706] (NBP3-25508) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

Immunohistochemistry-Paraffin: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] -

Immunohistochemistry-Paraffin: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] - hnRNP A2B1 antibody [HL1706] detects hnRNP A2B1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. hnRNP A2B1 stained by hnRNP A2B1 antibody [HL1706] (NBP3-25508) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] -

Immunocytochemistry/Immunofluorescence: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] - hnRNP A2B1 antibody [HL1706] detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A2B1 stained by hnRNP A2B1 antibody [HL1706] (NBP3-25508) diluted at 1:500. Blue: Fluoroshield with DAPI.
hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] -

Immunocytochemistry/Immunofluorescence: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] - hnRNP A2B1 antibody [HL1706] detects hnRNP A2B1 protein by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A2B1 stained by hnRNP A2B1 antibody [HL1706] (NBP3-25508) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

Western Blot: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] -

Western Blot: hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free [NBP3-25508] - Wild-type (WT) and HNRNPA2B1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody [HL1706] (NBP3-25508) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP A2B1

RNA polymerase II transcripts in the nucleus are in complex with several different proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins act in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA ranslation, and turnover.2. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform.3 The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weights (34-43 kDa).3 hnRNP-A2/B1 proteins are located in the nucleus of most tissues cells; however, the hnRNP-A2 protein is found also in the cytoplasm of skin and esophagus cells. In rat brain, hnRNP-A2/B1 proteins are found in neurons in the cerebral cortices, hippocampal formation, olfactory regions, caudate-putamin, and the supraoptic ucleus of the hypothalamus.4 Expression of hnRNP-A2/B1 proteins was found also in lung development and its over expression correlated with lung cancer.5

Alternate Names

DKFZp779B0244, FLJ22720, heterogeneous nuclear ribonucleoprotein A2/B1, heterogeneous nuclear ribonucleoprotein B1, heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2 / hnRNP B1, HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1hnRNP A2/B1, HNRPB1, nuclear ribonucleoprotein particle A2 protein, RNPA2, SNRPB1

Gene Symbol

HNRNPA2B1

Additional hnRNP A2B1 Products

Product Documents for hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for hnRNP A2B1 Antibody (HL1706) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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