hnRNP M1-M4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84555

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all hnRNP M1-M4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NKMGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23864636)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP M1-M4 Antibody - BSA Free

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555]

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555]

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555] - Staining of human lymph node shows strong nuclear positivity in lymphoid cells.
Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555]

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555]

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555] - Staining of human liver shows moderate nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555]

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555]

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555] - Staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555]

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555]

Immunohistochemistry-Paraffin: hnRNP M1-M4 Antibody [NBP1-84555] - Staining of human testis shows strong nuclear positivity in cells.
hnRNP M1-M4 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: hnRNP M1-M4 Antibody - BSA Free [NBP1-84555]

Immunocytochemistry/ Immunofluorescence: hnRNP M1-M4 Antibody - BSA Free [NBP1-84555]

Staining of human cell line A-431 shows localization to nucleoplasm.
hnRNP M1-M4 Antibody - BSA Free Western Blot: hnRNP M1-M4 Antibody - BSA Free [NBP1-84555]

Western Blot: hnRNP M1-M4 Antibody - BSA Free [NBP1-84555]

Analysis in human cell line HEK 293.
hnRNP M1-M4 Antibody - BSA Free Western Blot: hnRNP M1-M4 Antibody - BSA Free [NBP1-84555]

Western Blot: hnRNP M1-M4 Antibody - BSA Free [NBP1-84555]

Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HNRNPM antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
hnRNP M1-M4 Antibody - BSA Free Western Blot: hnRNP M1-M4 Antibody - BSA Free [NBP1-84555]

Western Blot: hnRNP M1-M4 Antibody - BSA Free [NBP1-84555]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for hnRNP M1-M4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP M1-M4

Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogenous nuclear ribonucleoproteins (hnRNPs), play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64kDa subunit of cleavage stimulation factor, which is involved in 3'-end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.

Alternate Names

DKFZp547H118, heterogeneous nuclear ribonucleoprotein M, hnRNP M, HNRNPM4, HNRPM4heterogenous nuclear ribonucleoprotein M, HNRPMheterogenous nuclear ribonucleoprotein M4, HTGR1, M4 protein, N-acetylglucosamine receptor 1, NAGR1hnRNA-binding protein M4

Gene Symbol

HNRNPM

Additional hnRNP M1-M4 Products

Product Documents for hnRNP M1-M4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for hnRNP M1-M4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for hnRNP M1-M4 Antibody - BSA Free

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Protocols

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