HO-1/HMOX1/HSP32 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31341

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HO-1/HMOX1/HSP32 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HO-1/HMOX1/HSP32. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (85%), Porcine (82%), Bovine (83%).

Localization

Microsome, Endoplasmic reticulum

Marker

Oxidative Stress Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HO-1/HMOX1/HSP32 Antibody - BSA Free

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341]

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341]

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Mouse liver. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341]

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341]

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Analysis of HO-1 in HEK293 cell lysate using anti-HO-1 antibody. WB Image submitted by a verified customer review.
Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341]

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341]

HO-1-HMOX1-HSP32-Antibody-Western-Blot-NBP1-31341-img0032.jpg
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341]

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341]

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Human renal carcinoma. Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341]

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341]

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
HO-1/HMOX1/HSP32 Antibody

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] -

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HO-1/HMOX1/HSP32 Antibody

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] -

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - HO-1/HMOX1/HSP32 antibody detects HO-1/HMOX1/HSP32 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
HO-1/HMOX1/HSP32 stained by HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HO-1/HMOX1/HSP32 Antibody

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] -

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HO-1/HMOX1/HSP32 Antibody

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] -

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
HO-1/HMOX1/HSP32 Antibody - BSA Free

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
HO-1/HMOX1/HSP32 Antibody - BSA Free

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HO-1/HMOX1/HSP32 Antibody - BSA Free

ELISA: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] -

Sandwich ELISA detection of recombinant full-length HO-1/HMOX1/HSP32 protein using GTX633677 as capture antibody at concentration of 5 ug/mL and NBP1-31341 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.

Applications for HO-1/HMOX1/HSP32 Antibody - BSA Free

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Sandwich ELISA

Assay dependent

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-31341 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HO-1/HMOX1/HSP32

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Long Name

Heme Oxygenase 1

Alternate Names

HMOX1, HO1, HSP32

Gene Symbol

HMOX1

Additional HO-1/HMOX1/HSP32 Products

Product Documents for HO-1/HMOX1/HSP32 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HO-1/HMOX1/HSP32 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HO-1/HMOX1/HSP32 Antibody - BSA Free

Customer Reviews for HO-1/HMOX1/HSP32 Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK-293 whole cell lysate
    Species: Human
    Verified Customer | Posted 03/20/2015
    HEK-293 cells lysates probed with HO-1
    HO-1/HMOX1/HSP32 Antibody - BSA Free NBP1-31341

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Protocols

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