HPV16 L1 Antibody (CamVir 1) - BSA Free

Novus Biologicals | Catalog # NB100-2732

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Human, Virus

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CamVir 1

Format

BSA Free
View all HPV16 L1 Primary Antibodies »

Product Specifications

Immunogen

A beta galactosidase-L1 fusion protein purified by PAGE.

Epitope

C terminus of HPV16 L1

Reactivity Notes

Human Papillomavirus (HPV)

Localization

Virion. Host nucleus.

Specificity

Cross reacts with HPV37. Reacts very strongly with biopsy specimins containing HPV16 or HPV33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV6 or HPV11.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for HPV16 L1 Antibody (CamVir 1) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 24231739)

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Knockdown Validated

reported in scientific literature (PMID 32210347)

Western Blot

1:100-1:2000
Application Notes
This HPV16 L1 (CamVir 1) antibody antibody reacts with a 56 kDa protein in cells infected with L1-vaccinia virus, the protein being present in a predominantly nuclear location.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPV16 L1

HPV16 L1, also known as HPV-16, Major capsid protein L1, and Major Capsid Protein is a papillomavirus. Papillomaviruses are small nonenveloped tumorigenic DNA viruses that infect the basal cells of the epithelium. Papillomavirions contain two proteins, L1 and L2, which encapsulate a closed, circular, double-stranded DNA. The major capsid protein L1 specifically binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. Thus, the location of HPV16 L1 is largely nuclear. Furthermore, stable interactions have been observed between L1 and the minor capsid protein L2, which is necessary for viral genome encapsulation. Virtually all cervical cancers are caused by human papilloma infections, with types 16 and 18 being responsible for about 70 percent of all cases. Currently, B-cell epitopes from type 16-L1 derived peptides are being investigated in order to gain a better understanding of immune responses to this virus (PMID: 22979950).

Alternate Names

HPV-16, HPV-16 capsid, HPV16 capsid protein, HPV16 L1, HPV16 major capsid protein L1, Human papillomavirus type 16 L1, Human papillomavirus type 16 major capsid protein L1, Major capsid protein, Major capsid protein L1

Entrez Gene IDs

1489082 (Human)

UniProt

P03101 (Human)

Additional HPV16 L1 Products

Product Documents for HPV16 L1 Antibody (CamVir 1) - BSA Free

Certificate of Analysis

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Product Specific Notices for HPV16 L1 Antibody (CamVir 1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HPV16 L1 Antibody (CamVir 1) - BSA Free

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Protocols

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