HSD17B4 Antibody (OTI4C4)

Novus Biologicals | Catalog # NBP2-46005

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Cited:

Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4C4
View all HSD17B4 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSD17B4(NP_000405) produced in HEK293T cell.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31176039). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HSD17B4 Antibody (OTI4C4)

Western Blot: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Western Blot: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Western Blot: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B4.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Carcinoma of Human liver tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human Ovary tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Carcinoma of Human pancreas tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human prostate tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Carcinoma of Human prostate tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human Kidney tissue.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human liver tissue.
Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4.
Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005]

Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of HeLa cells.

Applications for HSD17B4 Antibody (OTI4C4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunoprecipitation

Reported in scientific literature (PMID: 31176039).

Proximity Ligation Assay

Reported in scientific literature (PMID: 31176039).

Western Blot

1:4000

Reviewed Applications

Read 1 review rated 4 using NBP2-46005 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSD17B4

The protein encoded by the HSD17B4 gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathwayfor fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chainand 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidationactivity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present onchromosome 8. (provided by RefSeq)

Alternate Names

12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, 7-alpha, D-bifunctional protein, EDH17B4, hydroxysteroid (17-beta) dehydrogenase 4, MPF-2, peroxisomal, peroxisomal multifunctional enzyme type 2,17-beta-HSD IV, peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV, short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Gene Symbol

HSD17B4

Additional HSD17B4 Products

Product Documents for HSD17B4 Antibody (OTI4C4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HSD17B4 Antibody (OTI4C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HSD17B4 Antibody (OTI4C4)

Customer Reviews for HSD17B4 Antibody (OTI4C4) (1)

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  • HSD17B4 Antibody (4C4)
    Name: ZHU ZHIHUI
    Application: Western Blot
    Sample Tested: N2a and HEK293 T cell lysates
    Species: Mouse
    Verified Customer | Posted 02/01/2017
    We first test this antibody in HEK293T (Human Embryonic Kidney Cells) and N2a (mouse neuroblastoma cell line) cell lysis. Both of them show the positive bands when NBP2-46005 is used. Then we do the IP experiments, the bait is the interacting protein of HSD17B4 coated beads which are incubated with N2a cell lysates, we also set the control which is free of any interacting protein of HSD17B4. It can be detected in the blot that input and bait both show the positive bands of HSD17B4 but not control.
    HSD17B4 Antibody (OTI4C4) NBP2-46005

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