HSD17B4 Antibody (OTI4C4) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-70947
![Western Blot: HSD17B4 Antibody (OTI4C4)Azide and BSA Free [NBP2-70947]](https://resources.rndsystems.com/images/products/HSD17B4-Antibody-OTI4C4-Azide-and-BSA-Free-Western-Blot-NBP2-70947-img0001.jpg "Western Blot: HSD17B4 Antibody (OTI4C4)Azide and BSA Free [NBP2-70947]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4C4
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human HSD17B4(NP_000405) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HSD17B4 Antibody (OTI4C4) - Azide and BSA Free
Western Blot: HSD17B4 Antibody (OTI4C4)Azide and BSA Free [NBP2-70947]
Western Blot: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B4.![Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]](https://resources.rndsystems.com/images/products/HSD17B4-Antibody-OTI4C4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70947-img0009.jpg "Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]")
Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]
Immunocytochemistry/Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4.![Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]](https://resources.rndsystems.com/images/products/HSD17B4-Antibody-OTI4C4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70947-img0008.jpg "Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]")
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Human prostate tissue.![Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]](https://resources.rndsystems.com/images/products/HSD17B4-Antibody-OTI4C4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70947-img0010.jpg "Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]")
Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]
Immunocytochemistry/Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of HeLa cells.![Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]](https://resources.rndsystems.com/images/products/HSD17B4-Antibody-OTI4C4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70947-img0002.jpg "Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]")
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Carcinoma of Human liver tissue.![Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]](https://resources.rndsystems.com/images/products/HSD17B4-Antibody-OTI4C4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70947-img0003.jpg "Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]")
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Carcinoma of Human pancreas tissue.![Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]](https://resources.rndsystems.com/images/products/HSD17B4-Antibody-OTI4C4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70947-img0004.jpg "Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]")
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Carcinoma of Human prostate tissue.![Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]](https://resources.rndsystems.com/images/products/HSD17B4-Antibody-OTI4C4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70947-img0005.jpg "Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]")
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Human Kidney tissue.![Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]](https://resources.rndsystems.com/images/products/HSD17B4-Antibody-OTI4C4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70947-img0006.jpg "Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]")
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Human liver tissue.![Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]](https://resources.rndsystems.com/images/products/HSD17B4-Antibody-OTI4C4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70947-img0007.jpg "Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]")
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Human Ovary tissue.Applications for HSD17B4 Antibody (OTI4C4) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:4000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: HSD17B4
Alternate Names
12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, 7-alpha, D-bifunctional protein, EDH17B4, hydroxysteroid (17-beta) dehydrogenase 4, MPF-2, peroxisomal, peroxisomal multifunctional enzyme type 2,17-beta-HSD IV, peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV, short chain dehydrogenase/reductase family 8C, member 1,3-alpha
Gene Symbol
HSD17B4
Additional HSD17B4 Products
Product Documents for HSD17B4 Antibody (OTI4C4) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HSD17B4 Antibody (OTI4C4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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