HSP60 Antibody (LK2)

Novus Biologicals | Catalog # NBP2-32867

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Bacteria, S. pyogenes, Chicken, E. coli, Guinea Pig, Hamster, Invertebrate, Monkey, Parasite, Plant, Yeast

Cited:

Human, Mouse, Yeast

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot (Negative)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LK2
View all HSP60 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human HSP60 protein (Uniprot: P10809 )

Epitope

aa 383-419

Reactivity Notes

Borellia, Streptococcus pyogenes, Yersinia entercolitica, Salmonella typhimurium, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Helminths, and Spinach.

Localization

Mitochondrial

Marker

Mitochondrial Marker

Specificity

Recognizes a 60kDa protein, identified as the heat shock protein 60 (HSP60). Its epitope is localized between aa 383-419 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.

Antibody with azide - store at 2 to 8C.

Scientific Data Images for HSP60 Antibody (LK2)

Simple Western: HSP60 Antibody (LK2) [NBP2-32867]

Simple Western: HSP60 Antibody (LK2) [NBP2-32867]

Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: HSP60 Antibody (LK2) [NBP2-32867]

Western Blot: HSP60 Antibody (LK2) [NBP2-32867]

Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Western blot analysis of Hep2 cell lysate using HSP60 Mouse Monoclonal Antibody (LK2).
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (LK2) [NBP2-32867]

Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (LK2) [NBP2-32867]

Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK2) [NBP2-32867] - Immunofluorescent staining of MEOH-fixed HeLa cells using followed by goat anti-Mouse labeled with CF488 (Green); Nuclei are labeled with NucSpot red).
Immunohistochemistry-Paraffin: HSP60 Antibody (LK2) [NBP2-32867]

Immunohistochemistry-Paraffin: HSP60 Antibody (LK2) [NBP2-32867]

Immunohistochemistry-Paraffin: HSP60 Antibody (LK2) [NBP2-32867] - Formalin-fixed, paraffin-embedded human liver in colon stained with HSP60 Mouse Monoclonal Antibody (LK2).
Western Blot (Negative): HSP60 Antibody (LK2) [NBP2-32867]

Western Blot (Negative): HSP60 Antibody (LK2) [NBP2-32867]

HSP60-Antibody-LK2-Western-Blot-Negative-NBP2-32867-img0006.jpg
Simple Western: HSP60 Antibody (LK2) [NBP2-32867]

Simple Western: HSP60 Antibody (LK2) [NBP2-32867]

Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.
HSP60 Antibody (LK2)

Western Blot: HSP60 Antibody (LK2) [NBP2-32867] -

Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Mtb Rv1468c directly binds to poly-Ub chains in a UBA-dependent manner. a, b Immunoblot analysis of whole bacterial lysate of Mtb cells pretreated w/ (+) or w/out (–) proteinase K followed by incubation w/ K632–7 (a) or K482–7 (b) poly-Ub chains at 4 °C for 4 h. c Confocal microscopy analysis for colocalization of pSC301-Mtb stably expressing green fluorescent protein (GFP) w/ K632–7 or K482–7 Ub chains in vitro. Bacteria (green) treated w/ (+) or w/out (–) proteinase K further incubated w/ poly-Ub chains as in a & b, & then immunostained using anti-Ub antibody (red). Scale bars, 10 μm. The % colocalizations of Ub & Mtb shown at the bottom. A total of 100 bacterial cells counted. Two-tailed unpaired t-test performed. d Schematic representation of Mtb Rv1468c constructs used. e In vitro interaction analysis of Ub & GST-tagged full-length Mtb Rv1468c or the truncated mutants as indicated in d. Purified recombinant GST-tagged Mtb Rv1468c variants immobilized on glutathione sepharose resin. Mono-Ub (His6-tagged), K63 or K48-linked poly-Ub (Ub2–7) added, & then the protein complexes assayed by immunoblotting w/ indicated antibodies. f Immunoblot analysis of proteins immunoprecipitated (IP) w/ anti-Flag M2 Affinity Gel from lysates of HEK293T cells transfected w/ empty vector (–) or hemagglutinin (HA)-tagged Ub (+) & Flag-tagged wild-type (WT) Rv1468c or its mutants (V64G or L65G). g Confocal microscopy analysis of HeLa cells cotransfected w/ vectors encoding HA-tagged Ub (red) & GFP-tagged WT Rv1468c, V64G or L65G mutant (green). Nuclei (blue) stained w/ DNA-binding dye DAPI. Scale bars, 10 μm. Results representatives from at least 3 independent experiments (mean ± s.e.m. of n = 5 in c). The source data used in c provided in Source Data Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31036822), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for HSP60 Antibody (LK2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

1:10

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa and HepG2 lysates, separated by Size, antibody dilution of 1:10, apparent MW was 61 kDa.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HSP60

Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65 kDa major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1 billion years) and this fact together with the high degree of homology between the divergent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of magnesium ions and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single ring human mitochondrial homolog, Hsp60 with its co chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co chaperonin, GroES. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase 3 by cytochrome c and dATP in an ATP dependent manner. Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, arthrosclerosis and chlamydial disease.

Long Name

Heat Shock Protein 60

Alternate Names

cpn60, GROEL, HSPD1, SPG13

Gene Symbol

HSPD1

UniProt

P10809 (Mouse)

Additional HSP60 Products

Product Documents for HSP60 Antibody (LK2)

Certificate of Analysis

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Product Specific Notices for HSP60 Antibody (LK2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

Citations for HSP60 Antibody (LK2)

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Protocols

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