HspA1B Antibody (3B7) - Azide and BSA Free

Western Blot: HspA1B Antibody (3B7) [H00003304-M02]

Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7. Analysis of HSPA1B expression in PC-12.

Western Blot: HspA1B Antibody (3B7) [H00003304-M02]

Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7 Analysis of HSPA1B expression in IMR-32.

Western Blot: HspA1B Antibody (3B7) [H00003304-M02]

Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7. Analysis of HSPA1B expression in Raw 264.7.

Immunocytochemistry/ Immunofluorescence: HspA1B Antibody (3B7) [H00003304-M02]

Immunocytochemistry/Immunofluorescence: HspA1B Antibody (3B7) [H00003304-M02] - Analysis of monoclonal antibody to HSPA1B on HeLa cell. Antibody concentration 10 ug/ml.

Proximity Ligation Assay: HspA1B Antibody (3B7) [H00003304-M02]

Proximity Ligation Assay: HspA1B Antibody (3B7) [H00003304-M02] - Analysis of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-HSPA1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

ELISA: HspA1B Antibody (3B7) [H00003304-M02]

ELISA: HspA1B Antibody (3B7) [H00003304-M02] - Detection limit for recombinant GST tagged HSPA1B is approximately 0.03ng/ml as a capture antibody.

Immunocytochemistry/ Immunofluorescence: HspA1B Antibody (3B7) [H00003304-M02] -

Immunocytochemistry/ Immunofluorescence: HspA1B Antibody (3B7) [H00003304-M02] - Validation of the LCM/MS data from cellular protrusions using microscopy. Immunofluorescence (IF) of proteins identified by LCM/MS (A–D) “expected” or (E–H) “not expected” to be within protrusions based on their known functions/localizations. (A) Cd47 & (B) Anxa2 are found in both hCAD/dCAD protrusions. (C) Tenm2 is found within hCAD protrusions & (D) Cobl in GCs. Interestingly, (E) Grk5 was observed in hCAD protrusions; (F) Hist1h3b protein in hCAD protrusions & GCs (G) Hspa1b in dCAD protrusions & TNTs & (H) Arg1 in dCAD protrusions, GCs, & TNTs. White arrows/arrowheads show punctates within dCAD/hCAD protrusions & GCs or within TNTs, respectively. Scale bars = 10 μm. IF of all 8 proteins corroborate the LCM/MS protein identification for each subtypes of protrusions. (I) 1198 unique proteins were identified by LCM/MS to be in protrusions & 169 were found to be exclusive to protrusions. 904 of the 1198 proteins found in protrusions & 87 out of 169 exclusive proteins had images in the HPA Subcell database. IF images for the proteins identified by LCM/MS in protrusions, present in the HPA Subcell database, were observed & catalogued as being observed or not in protrusions (Figure S2). On average 4.7% of proteins are found in protrusions in the HPA database, 13.6% are found in our LCM isolated protrusions & 23% were found in the “exclusive” LCM protrusions. The fold enrichment increases by 2.9% or 4.8% when we looked at proteins identified by LCM/MS in protrusions or exclusive to protrusions, respectively. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30866487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.