Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Guinea Pig, Hamster, Monkey, Rabbit, Sheep

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Electron Microscopy

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 1B5
View all HSPA8/HSC71 Primary Antibodies »

Product Specifications

Immunogen

Native hamster Hsc70 (Hsp73).

Reactivity Notes

Turtle (100%).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for HSPA8/HSC71/Hsc70 Antibody (1B5) [Biotin]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [Biotin] [NBP1-97876] - Analysis of HSC70/HSP73, mAb (1B5) (biotin conjugate): Lane 1: HSC70/HSP73 (bovine), (rec); Lane 2: HSP70/HSP72 (human), (recombinant); Lane 3: 3T3 (heat shocked): Lane 4: PC-12 (heat shocked); Lane 5: CHO-K1 (heat shocked); Lane 6: GPC-16 (heat shocked); Lane 7: HeLa (heat shocked); Lane 8: Vero (heat shocked).

Applications for HSPA8/HSC71/Hsc70 Antibody (1B5) [Biotin]

Application
Recommended Usage

ELISA

1:100-1:2000

Electron Microscopy

1:10-1:500

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
This antibody is useful in Electron Electron Microscopy, Enzyme Immunoassay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation and detects a band of ~73kDa by Western blot.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSPA8/HSC71

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Long Name

Heat Shock 70 kDa Protein 8

Alternate Names

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, NIP71

Gene Symbol

HSPA8

Additional HSPA8/HSC71 Products

Product Documents for HSPA8/HSC71/Hsc70 Antibody (1B5) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HSPA8/HSC71/Hsc70 Antibody (1B5) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

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Protocols

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