HSPA8/HSC71/Hsc70 Antibody (1F2-H5) - BSA Free

Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880]

Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.

Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) - BSA Free [NBP2-12880] -

RNAi screening of host factors related to EBOV-trVLP life cycle and further validation and analysis. (A) RNAi silencing analysis of host factors required for the EBOV trVLP life cycle. A 100 μl sample of opti-MEM medium containing 1.4 μl siRNA and 4.5 μl HiPerFect was placed in 24-well plates, and a cell suspension (400 μl) containing 1 × 105 cells was added to give a final siRNA concentration of 75 nM. After incubation for 48 h, cells were infected with trVLPs for another 48 h, total RNA was extracted, and the absolute quantity of EBOV RNA was measured using a EBOV nucleic acid test kit. All qRT-PCR experiments were performed in triplicate and repeated three times independently. Cells not transfected with siRNA but infected with trVLPs served as a blank control; cells transfected with isotype siRNA and infected with trVLPs served as a negative control. Eleven siRNAs targeting HSPA1A, HSP90AB1, ARFGAP1, ANXA5, YWHAZ, MAPK11, NTRK1, FLT4, GRP78, MEK2, and HSPA8 inhibited trVLP replication effectively. ∗p < 0.05, ∗∗p < 0.01. (B) Western blot analysis of each target protein after siRNA transfection. The 11 target proteins were all expressed at much lower levels following transfection with the relevant siRNA. Normal 293T cells and cells transfected with isotype siRNAs served as controls. (C) Characterization of selected networks of candidate host proteins that may be important for regulating the trVLP life cycle. Interactions of the 11 host proteins were assessed using the GeneMANIA interaction database, and their functions were evaluated in association with cytoplasmic vesicle membranes, mitochondrial membranes, mitochondria, antigen binding, the COP9 signalosome, and phospholipid binding. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30483236), licensed under a CC-BY license. Not internally tested by Novus Biologicals.