HTR12 Antibody

Novus Biologicals | Catalog # NBP1-18694

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

A. thaliana, Other, Plant

Cited:

A. thaliana, Other

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all HTR12 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide derived from Nter domain of Arabidopsis thaliana (Mouse-ear cress) HTR12

Reactivity Notes

Other reactivity reported in scientific literature (PMID: 26124146). Arabidopsis thaliana. (Also known as Mouse-ear cress.) Use in A. thaliana reported in secitific publication PMID: 32572214

Specificity

Reacts specifically with 17 kDa protein of HTR12

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for HTR12 Antibody

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:50-1:500

Western Blot

1:200-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Lyophilized powder, reconstituted in distilled water.

Formulation

Antiserum

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HTR12

HTR12 is a histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation and may serve as an epigenetic mark that propagates centromere identity through replication and cell division.

Alternate Names

CENH3, Centromeric histone CENH3, F6F3.17, F6F3_17, Histone H3 like centromeric protein HTR12, HTR 12

Entrez Gene IDs

1058 (Human)

Gene Symbol

HTR12

UniProt

P49450 (Human)

Additional HTR12 Products

Product Documents for HTR12 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HTR12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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