Human ADAM12 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (2)
Supplemental Products
Reviews (1)

Human ADAM12 Antibody Summary

Species Reactivity
Detects human ADAM12 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human ADAM8, 9, 10, 15, 17, 19, 22, 23, 32, or 33 is observed.
Monoclonal Mouse IgG1 Clone # 632525
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human ADAM12
Accession # O43184
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAM12 (Catalog # 4416-AD), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM12

ADAM12 (A disintegrin and metalloprotease domain 12; also Meltrin-a) is a 120 kDa type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909 amino acid (aa) preproprecursor that contains a 25 kDa proregion (aa 29-207) and a 92 kDa mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression.  A 68 kDa soluble splice form exists in pregnancy where 36 amino acids replace the C-terminal 205 aa (705-909).  This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator.  Over aa 29-513, human ADAM12 shares 78% and 79% aa identity with bovine and mouse ADAM12, respectively.

Long Name
A Disintegrin and Metalloprotease-like Domain 12
Entrez Gene IDs
8038 (Human)
Alternate Names
a disintegrin and metalloproteinase domain 12 (meltrin alpha); ADAM 12; ADAM metallopeptidase domain 12; ADAM12; disintegrin and metalloproteinase domain-containing protein 12; EC 3.4.24; EC; MCMP; MCMPMltna; Meltrin alpha; meltrin-alpha; MLTNA; MLTNEC 3.4.24.-

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Citations for Human ADAM12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Metalloprotease-disintegrin ADAM12 actively promotes the stem cell-like phenotype in claudin-low breast cancer
    Authors: S Duhachek-M, Y Qi, R Wise, L Alyahya, H Li, J Hodge, A Zolkiewska
    Mol. Cancer, 2017;16(1):32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Phenotypic diversity of breast cancer-related mutations in metalloproteinase-disintegrin ADAM12.
    Authors: Qi, Yue, Duhachek-Muggy, Sara, Li, Hui, Zolkiewska, Anna
    PLoS ONE, 2014;9(3):e92536.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Reconstitution Buffers

Secondary Antibodies

Reviews for Human ADAM12 Antibody

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Human ADAM12 Antibody
By Anonymous on 07/06/2021
Application: ELISA Sample Tested: Serum and Plasma Species: Human

we used this antibody for an ELISA to quantify human ADAM12 in serum, which works very well.