Human ADAM12 Antibody

Name: Human ADAM12 Antibody
Brand: R&D Systems
SKU: MAB44161-SP
Rating: 5 (1 reviews)

Catalog #: MAB44161
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB44161
MAB44161-SP

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Citations (2)

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Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM12 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAM12 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human ADAM8, 9, 10, 15, 17, 19, 22, 23, 32, or 33 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 632525

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM12
Arg29-Ser513
Accession # O43184

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ADAM12 (Catalog # 4416-AD), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM12

ADAM12 (A disintegrin and metalloprotease domain 12; also Meltrin-a) is a 120 kDa type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909 amino acid (aa) preproprecursor that contains a 25 kDa proregion (aa 29-207) and a 92 kDa mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression. A 68 kDa soluble splice form exists in pregnancy where 36 amino acids replace the C-terminal 205 aa (705-909). This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator. Over aa 29-513, human ADAM12 shares 78% and 79% aa identity with bovine and mouse ADAM12, respectively.

Long Name

A Disintegrin and Metalloprotease-like Domain 12

Entrez Gene IDs

8038 (Human)

Alternate Names

a disintegrin and metalloproteinase domain 12 (meltrin alpha); ADAM 12; ADAM metallopeptidase domain 12; ADAM12; disintegrin and metalloproteinase domain-containing protein 12; EC 3.4.24; EC 3.4.24.58; MCMP; MCMPMltna; Meltrin alpha; meltrin-alpha; MLTNA; MLTNEC 3.4.24.-

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human ADAM12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Metalloprotease-disintegrin ADAM12 actively promotes the stem cell-like phenotype in claudin-low breast cancer
Authors: S Duhachek-M, Y Qi, R Wise, L Alyahya, H Li, J Hodge, A Zolkiewska
Mol. Cancer, 2017;16(1):32.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phenotypic diversity of breast cancer-related mutations in metalloproteinase-disintegrin ADAM12.
Authors: Qi, Yue, Duhachek-Muggy, Sara, Li, Hui, Zolkiewska, Anna
PLoS ONE, 2014;9(3):e92536.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry