Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ADAM12 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM12
Arg29-Ser513
Accession # AAC08702

Specificity

Detects human ADAM12 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) ADAM8, rhADAM19, and recombinant mouse ADAM33 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human ADAM12 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM12 (Catalog # 4416‑AD), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAM12 (Catalog # 4416‑AD)

Reviewed Applications

Read 1 review rated 5 using AF4416 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM12

ADAM12, also known as meltrin-alpha, is a member of the ADAM family with metalloprotease activity (1). It consists of a propeptide, metalloproteinase, disintegrin, cysteine-rich, and EGF-like domains, a transmembrane segment, and a cytoplasmic tail with SH3 binding motifs. Human ADAM12 exists in two alternatively spliced forms: the prototype transmembrane form and a shorter secreted form lacking the transmembrane domain and the cytoplasmic tail. The secreted form has a 34 amino acid substitution in place of the transmembrane and cytoplasmic regions. In mouse, only the transmembrane form has been observed. The propeptide, which is cleaved in the Golgi by furin-like proprotein convertases, is retained in a noncovalent complex after ADAM12 secretion (2). Thus, the pro domain may function as an inhibitor of the proteolytic activity or play another unknown function. The known physiological substrates of ADAM12 are HBEGF in the heart (3) and IGFBP-3 and -5 in placental serum (4). Its proteolytic activity is inhibited by the tissue inhibitor of metalloproteinase-3 (recombinant human TIMP-3, Catalog # http://www.rndsystems.com/product_results.aspx?k=973-TM">973‑TM) and alpha ‑2‑macroglobulin. It also mediates cell‑cell adhesion by interacting with integrins and syndecans as well as with additional unidentified molecules (4). ADAM12 may be a promising marker in prenatal diagnostics and breast cancer (5, 6). The recombinant ADAM12 contains the pro, metalloproteinase, and disintegrin domains. In addition to TIMP-3, the activity can also be inhibited by 5 mM 1,10-phenanthroline.

References

  1. Wewer, U.M. (2004) in Handbook of Proteolytic Enzymes, Barrett, A. J. et al. eds. pp 724.
  2. Wewer, U.M. et al. (2006) J. Biol. Chem. 281:9481.
  3. Asakura, M. et al. (2002) Nat. Med. 8:35.
  4. Loechel, F. et al. (2000) Biochem. Biophys. Res. Comm. 278:511.
  5. Laigaard, J. et al. (2006) Prenat Diagn. 26:973.
  6. Roopali, R. et al. (2004) J. Biol. Chem. 279:51323.

Long Name

A Disintegrin and Metalloprotease-like Domain 12

Alternate Names

Meltrin alpha

Entrez Gene IDs

8038 (Human)

Gene Symbol

ADAM12

UniProt

AAC08702

Additional ADAM12 Products

Product Documents for Human ADAM12 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ADAM12 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for Human ADAM12 Antibody (1)

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  • Human ADAM12 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/06/2021
    we used this antibody for an ELISA to quantify human ADAM12 in serum, which works very well.
    Human ADAM12 Antibody AF4416

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