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Human ADAM15 Ectodomain PE-conjugated Antibody

Catalog #: IC935P Datasheet / COA / SDS
Newer Version Available: IC935N
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Discontinued Product

IC935P has been discontinued and is replaced by IC935N.

Detection of ADAM15 in MCF‑7 Human Cell Line by Flow Cytometry.
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Human ADAM15 Ectodomain PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross‑reactivity with recombinant mouse (rm) ADAM15 but does not cross-react with rhADAM8, 9, 17, 28, rmADAM9, or 10.
Source
Monoclonal Mouse IgG1 Clone # 23G9
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human ADAM15
Asp207-Thr696
Accession # Q13444
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of ADAM15 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ADAM15 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ADAM15 Ectodomain PE-conjugated Monoclonal Antibody (Catalog # IC935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ADAM15

ADAM15, also known as MDC15, Disintegrin and Metallo- and Proteinase Domain-containing Protein 15, and Metargidin, is a member of a family of transmembrane proteins that contain disintegrin and metalloprotease domains. It is synthesized as a 110 kDa, type I transmembrane proform that may be processed into a 75 kDa form that contains the metalloprotease plus disintegrin domain. Further processing may release the 26 kDa metalloproteinase domain, extending proteinase activity beyond the cell membrane. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Long Name
A Disintegrin and Metalloprotease-like Domain 15
Entrez Gene IDs
8751 (Human); 11490 (Mouse)
Alternate Names
ADAM 15; ADAM metallopeptidase domain 15; ADAM15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin

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