Human ADAMTS13 Quantikine ELISA Kit

R&D Systems | Catalog # DADT130

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)

Sensitivity

0.26 ng/mL

Assay Range

0.781-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
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Product Summary for Human ADAMTS13 Quantikine ELISA Kit

The Quantikine Human ADAMTS13 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ADAMTS13 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human ADAMTS13 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ADAMTS13 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ADAMTS13.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human ADAMTS13. This kit also recognizes a truncated version of recombinant human ADAMTS13 (amino acids Gln34-Trp688; Accession # NP_620594).

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.78 13.8 29.7 4.51 14.2 30.2
Standard Deviation 0.177 0.364 0.685 0.256 0.722 1.08
CV% 3.7 2.6 2.3 5.7 5.1 3.6

Recovery for Human ADAMTS13 Quantikine ELISA Kit

The recovery of ADAMTS13 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 100 91-111
Citrate Plasma (n=4) 97 87-114
EDTA Plasma (n=4) 97 89-109
Heparin Plasma (n=4) 97 85-115
Serum (n=4) 97 85-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ADAMTS13 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human ADAMTS13 ELISA Linearity

Scientific Data Images for Human ADAMTS13 Quantikine ELISA Kit

Human ADAMTS13 ELISA Standard Curve

Human ADAMTS13 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ADAMTS13

Members of the ADAMTS family contain pro, catalytic, disintegrin-like, cysteine-rich, spacer and a variable number of TS type 1 motifs. In contrast to membrane-anchored ADAMs, ADAMTSs are secreted and are all active proteases. They are essential for aggrecan turnover and procollagen processing, regulation of which is important in many physiological and pathological processes such as angiogenesis and arthritis.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13

Alternate Names

TTP, vWF-CP

Entrez Gene IDs

11093 (Human); 279028 (Mouse); 362091 (Rat)

Gene Symbol

ADAMTS13

Additional ADAMTS13 Products

Product Documents for Human ADAMTS13 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human ADAMTS13 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human ADAMTS13 Quantikine ELISA Kit

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    Sample Tested: EDTA Plasma
    Verified Customer | Posted 08/06/2024
    Human ADAMTS13 Quantikine ELISA Kit DADT130
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    Sample Tested: Serum
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Protocols

View specific protocols for Human ADAMTS13 Quantikine ELISA Kit (DADT130):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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