Human Adenosylhomocysteinase/AHCY Antibody

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Detection of Human Adenosylhomocysteinase/AHCY by Western Blot.
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Product Details
Citations (1)
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Human Adenosylhomocysteinase/AHCY Antibody Summary

Species Reactivity
Detects human Adenosylhomocysteinase/AHCY in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human Adenosylhomocysteinase/AHCY
Accession # P23526
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Adenosylhomocysteinase/AHCY antibody by Western Blot. View Larger

Detection of Human Adenosylhomocysteinase/AHCY by Western Blot. Western blot shows lysates of Bowes human melanoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Adenosylhomocysteinase/AHCY Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosylhomocysteinase/AHCY at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosylhomocysteinase/AHCY

Human S‑Adenosylhomocysteinase (AHCY) is a cytoplasmic tetramer with a tightly bound NAD co‑factor for each subunit (1, 2). It is the only known enzyme to catalyze the breakdown of S‑adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCY’s activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease (3). It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S‑adonosyl-L-methionine methyltransferases (1). A mutation in the human AHCY results in AHCY deficiency with increase of plasma creatine kinase, methionine, S‑adenosylmethionine and AdoHcy, delay of myelination, myopathy and psychomotor retardation (4, 5).

  1. Turner, M. A. et al. (2000) Cell Biochem. Biophys. 33:101.
  2. Takata, Y. et al. (2002) J. Biol. Chem. 277:22670.
  3. Gellekink, H. et al. (2004) Eur. J. Hum. Genet. 12:942.
  4. Baric, I. et al. (2004) Proc. Natl. Acad. Sci. USA. 101:4234.
  5. Fumic, K. et al. (2007) Eur. J.Hum. Genet. 15:347. 
Entrez Gene IDs
191 (Human)
Alternate Names
Adenosylhomocysteinase; AdoHcyase; AHCY; EC; S-adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase; SAHH; SAHHadoHcyase

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Citation for Human Adenosylhomocysteinase/AHCY Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of cellular methyltransferases promotes endothelial cell activation by suppressing glutathione peroxidase 1 protein expression.
    Authors: Barroso M, Florindo C, Kalwa H, Silva Z, Turanov A, Carlson B, de Almeida I, Blom H, Gladyshev V, Hatfield D, Michel T, Castro R, Loscalzo J, Handy D
    J Biol Chem, 2014;289(22):15350-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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