Human Aldo-keto Reductase 1C4/AKR1C4 Antibody

Catalog # Availability Size / Price Qty
AF6957
AF6957-SP
Detection of Human Aldo‑keto Reductase 1C4/AKR1C4 by Western Blot.
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Human Aldo-keto Reductase 1C4/AKR1C4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Aldo-keto Reductase 1C4/AKR1C4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) AKR-1C1 and rhAKR-1C3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Aldo-keto Reductase 1C4/AKR1C4
Met1-Tyr323
Accession # NP_001809
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Aldo‑keto Reductase 1C4/AKR1C4 by Western Blot. View Larger

Detection of Human Aldo‑keto Reductase 1C4/AKR1C4 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Aldo‑keto Reductase 1C4/AKR1C4 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF6957) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldo‑keto Reductase 1C4/AKR1C4 at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aldo-keto Reductase 1C4/AKR1C4

AKR1C4 (Aldo-Keto Reductase family 1 member C4; also 3-alpha HSD1/3-alpha HSD type 1, Chlordecone/kepone reductase/CDR and DD4) is a monomeric, 36-38 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. AKRs catalyse the reduction of aldehydes and ketones into alcohols through a NADPH-dependent process. The resulting alcohols become the target of subsequent conjugation reactions. AKR1C4 in particular is noted for its action on dihydroxytestosterone, converting it to a less active 3 alpha -diol. In addition, it also converts progesterone into 20 alpha -hydroxyprogesterone, a molecule that both induces GnRH release, and blocks the generation of (deoxy)cortisol from 17 alpha -hydroxyprogesterone. Finally, AKR1C4 likely is involved in the tetoxification of xenobiotics and drugs. AKR1C4 expression is essentially limited to hepatocytes and likely breast epithelium. Human AKR1C4 is 323 amino acids (aa) in length. Full-length human AKR1C4 shares 83% and 76% aa sequence identity with human AKR1C1 and mouse AKR1C4, respectively.

Long Name
Aldo-keto Reductase Family 1, Member C4
Entrez Gene IDs
1109 (Human)
Alternate Names
3-alpha-HSD; AKR1C4; Aldo-keto Reductase 1C4; aldo-keto reductase family 1, member C4; AldoketoReductase 1C4; C11; CDR; CHDR; DD4; DD-4; HAKRA

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