Detection of Human Aldo‑keto Reductase 1C4/AKR1C4 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Aldo‑keto Reductase 1C4/AKR1C4 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF6957) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldo‑keto Reductase 1C4/AKR1C4 at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aldo-keto Reductase 1C4/AKR1C4
AKR1C4 (Aldo-Keto Reductase family 1 member C4; also 3-alpha HSD1/3-alpha HSD type 1, Chlordecone/kepone reductase/CDR and DD4) is a monomeric, 36-38 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. AKRs catalyse the reduction of aldehydes and ketones into alcohols through a NADPH-dependent process. The resulting alcohols become the target of subsequent conjugation reactions. AKR1C4 in particular is noted for its action on dihydroxytestosterone, converting it to a less active 3 alpha -diol. In addition, it also converts progesterone into 20 alpha -hydroxyprogesterone, a molecule that both induces GnRH release, and blocks the generation of (deoxy)cortisol from 17 alpha -hydroxyprogesterone. Finally, AKR1C4 likely is involved in the tetoxification of xenobiotics and drugs. AKR1C4 expression is essentially limited to hepatocytes and likely breast epithelium. Human AKR1C4 is 323 amino acids (aa) in length. Full-length human AKR1C4 shares 83% and 76% aa sequence identity with human AKR1C1 and mouse AKR1C4, respectively.
Aldo-keto Reductase Family 1, Member C4
Entrez Gene IDs:
3-alpha-HSD; AKR1C4; Aldo-keto Reductase 1C4; aldo-keto reductase family 1, member C4; AldoketoReductase 1C4; C11; CDR; CHDR; DD4; DD-4; HAKRA
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