Detection of Human alpha 1B-Glycoprotein by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7757) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha 1B-Glycoprotein at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human alpha 1B-Glycoprotein by Simple WesternTM.
Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for alpha 1B-Glycoprotein at approximately 94 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7757) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1B-Glycoprotein
A1BG (alpha-1 B/beta-glycoprotein; also C44 [in rodent]) is a 68-80 kDa monomeric plasma glycoprotein member of the Immunoglobulin superfamily of molecules. It is expressed by multiple cell types, principally hepatocytes in response to circulating growth hormone. Initially thought to act as an MMP inhibitor, A1BG is now known to bind to CRISP3, a distant member of a family of venomous molecules. CRISP3 is widely expressed and possesses no toxic properties. But it may play a key role in fertilization, where it temporarily blocks the reaction of PMNs to sperm in the uterus, thus increasing sperm lifespan and the likelihood of fertilization. Within this context, A1BG may regulate CRISP3 availability. A1BG has also been investigated as a biomarker in various cancers. Mature human A1BG is 474 amino acids (aa) in length (aa 22-495) and contains five V-type Ig-like domains. There is one isoform variant that utilizes Met123 as an alternative start site. Full-length human A1BG (aa 22-495) shares only 44% aa sequence identity with mouse A1BG.
Entrez Gene IDs:
1 (Human); 117586 (Mouse)
A1B; A1BG; ABG; alpha 1BGlycoprotein; alpha 1B-Glycoprotein; GAB
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