Human alpha-Aminoadipate Aminotransferase Antibody Summary
Met1-Leu425
Accession # Q8N5Z0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example

Detection of Human alpha -Aminoadipate Aminotransferase by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Aminoadipate Aminotransferase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7927) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Aminoadipate Aminotransferase at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Aminoadipate Aminotransferase
Alpha-Aminoadipate Aminotransferase (AADAT, KAT2 and Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial) is a 47 kDa (predicted) mitochondrial member of the class-I pyridoxal-phosphate-dependent aminotransferase family of proteins. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. Human ADDAT is predominantly expressed in the liver, it is also found in heart, brain, kidney, pancreas, prostate, testis and ovary. Over aa 1-425, human AADAT shares 73% aa sequence identity with mouse ADDAT.
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