Human alpha-Aminoadipate Aminotransferase Antibody

AF7927 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7927
AF7927-SP

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Human alpha-Aminoadipate Aminotransferase Antibody in Western Blot

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha-Aminoadipate Aminotransferase Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha -Aminoadipate Aminotransferase in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human alpha -Aminoadipate Aminotransferase
Met1-Leu425
Accession # Q8N5Z0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Human alpha -Aminoadipate Aminotransferase by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Aminoadipate Aminotransferase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7927) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Aminoadipate Aminotransferase at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Aminoadipate Aminotransferase

Alpha-Aminoadipate Aminotransferase (AADAT, KAT2 and Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial) is a 47 kDa (predicted) mitochondrial member of the class-I pyridoxal-phosphate-dependent aminotransferase family of proteins. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. Human ADDAT is predominantly expressed in the liver, it is also found in heart, brain, kidney, pancreas, prostate, testis and ovary. Over aa 1-425, human AADAT shares 73% aa sequence identity with mouse ADDAT.

Long Name

alpha-Aminoadipate Aminotransferase

Entrez Gene IDs

51166 (Human); 23923 (Mouse); 29416 (Rat)

Alternate Names

AADAT; alphaAminoadipate Aminotransferase; alpha-Aminoadipate Aminotransferase; aminoadipate aminotransferase; EC 2.6.1.39,2-aminoadipate aminotransferase; EC 2.6.1.7,2-aminoadipate transaminase; KAT/AadAT; KAT2; KAT2mitochondrial; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase II; L kynurenine/alpha aminoadipate aminotransferase