Human alpha-Defensin 1 DuoSet ELISA

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Human alpha-Defensin 1 ELISA Standard Curve
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Product Details
Citations (5)
Supplemental Products

Human alpha-Defensin 1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
0.5 - 32 ng/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human alpha-Defensin 1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Human alpha-Defensin 1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Wound alpha defensin levels are significantly higher in patients with fracture related infection: A pilot, prospective cohort study
    Authors: P Kumar, A Mittal, P Bansal, S Aggarwal, RK Rajnish, A Dadra, S Sharma
    Injury, 2022-12-13;0(0):.
    Species: Human
    Sample Types: Wound Fluid
  2. Metal Artefact Reduction Sequences (MARS) in Magnetic Resonance Imaging (MRI) after Total Hip Arthroplasty (THA) : A non-invasive approach for preoperative differentiation between periprosthetic joint infection (PJI) and aseptic complications?
    Authors: A Busch, M Jäger, S Beck, A Wegner, E Portegys, D Wassenaar, J Theysohn, J Haubold
    Bmc Musculoskeletal Disorders, 2022-06-28;23(1):620.
    Species: Human
    Sample Types: Synovial Fluid
  3. Decreased leukocyte exhaustion is associated with decreased IFN-beta and increased alpha-defensin-1 levels in type-2 diabetes
    Authors: S Shruthi, JM Sibi, V Mohan, S Babu, V Nirmaladev, V Aravindhan
    Cytokine, 2022-05-28;156(0):155918.
    Species: Human
    Sample Types: Serum
  4. Development of a multiplex and sensitive lateral flow immunoassay for the diagnosis of periprosthetic joint infection
    Authors: TT Tsai, TH Huang, NY Ho, YP Chen, CA Chen, CF Chen
    Sci Rep, 2019-10-30;9(1):15679.
    Species: Human
    Sample Types: Synovial Fluid
  5. 20-Hydroxy- and 20-carboxy-leukotriene (LT) B4 downregulate LTB4 -mediated responses of human neutrophils and eosinophils
    Authors: AS Archambaul, S Poirier, JS Lefebvre, PP Robichaud, MC Larose, C Turcotte, C Martin, V Provost, LH Boudreau, PP McDonald, M Laviolette, ME Surette, N Flamand
    J. Leukoc. Biol., 2019-01-24;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates


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