Human alpha ‑N‑acetylgalactosaminidase/NAGA Antibody

Catalog # Availability Size / Price Qty
Detection of Human  alpha ‑N‑acetylgalactosaminidase/NAGA by Western Blot.
1 Image
Product Details
Supplemental Products

Human alpha ‑N‑acetylgalactosaminidase/NAGA Antibody Summary

Species Reactivity
Detects human alpha ‑N‑acetylgalactosaminidase/NAGA in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human alpha -Galactosidase A is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line recombinant human alpha ‑N‑acetylgalactosaminidase/NAGA
Accession # P17050
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human a-N-acetylgalactosaminidase/NAGA antibody by Western Blot. View Larger

Detection of Human alpha ‑N‑acetylgalactosaminidase/NAGA by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-N-acetylgalactosaminidase/NAGA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6717) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-N-acetylgalactosaminidase/NAGA at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-N-acetylgalactosaminidase/NAGA

NAGA is a lysosomal alpha -N-acetylgalactosaminidase that cleaves non-reducing alpha -N-acetylgalactosaminyl moieties from glycoconjugates (1). Mature NAGA has 394 amino acids and is trafficked to the lysosome via the mannose-6-phosphate receptor‑mediated pathway (2). The enzyme is a retaining exoglycosidase, where both the substrate and product of the enzymatic reaction have the same anomeric configuration (3). Deficiency in NAGA results in increased urinary excretion and tissue accumulation of glycopeptides and oligosaccharides containing terminal alpha ‑N‑acetylgalactosaminyl moieties (4), manifesting as Schindler’s disease, an autosomal recessive disease with neuroaxonal dystrophy and other neurological symptoms (5). The enzyme can be used to remove alpha ‑N‑acetylgalactosaminyl residues present on red blood cells thus converting blood type A to blood type O (6, 7, 8).

  1. Wang, A.M. et al. (1990) J. Biol. Chem. 265:21859.
  2. Sweeley, C.C. et al. (1983) Arch. Biochem. Biophys. 223:158.
  3. Garman, S.C. et al. (2002) Structure. 10:425.
  4. Eng, C.M. et al. (2001) N. Engl. J. Med. 345:9.
  5. Wang, A.M. et al. (1990) J. Clin. Invest. 86:1752.
  6. Liu, Q.P. et al. (2007) Nature Biotechnol. 25:454.
  7. Calcutt, M. J. et al. (2002) FEMS Microbiol. Lett. 214:77.
  8. Zhu, A. et al. (1996) Arch. Biochem. Biophys. 327:324.
Entrez Gene IDs
4668 (Human); 17939 (Mouse); 315165 (Rat)
Alternate Names
alpha-N- (alpha-galactosidase B); alphaNacetylgalactosaminidase; alpha-N-acetylgalactosaminidase; EC 3.2.1; EC; GALB; N-acetylgalactosaminidase, alpha-; NAGA

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human alpha ‑N‑acetylgalactosaminidase/NAGA Antibody

There are currently no reviews for this product. Be the first to review Human alpha ‑N‑acetylgalactosaminidase/NAGA Antibody and earn rewards!

Have you used Human alpha ‑N‑acetylgalactosaminidase/NAGA Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review