Human alpha-Parvin Antibody

Catalog # Availability Size / Price Qty
AF5556
AF5556-SP
Detection of Human  alpha -Parvin by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human alpha-Parvin Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha -Parvin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human beta -Parvin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human alpha -Parvin
Val46-Phe133
Accession # Q9NVD7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human  alpha -Parvin by Western Blot. View Larger

Detection of Human alpha -Parvin by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human alpha -Parvin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5556) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha -Parvin at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Parvin

alpha -Parvin (parvus [Latin for "small"]-A/ alpha ; also actopaxin and PARV-A) is a 53 kDa member of the parvin family, alpha ‑actinin superfamily of proteins. It is widely expressed, and participates in actin filament assembly that precedes cell migration. Human PARVA is 372 amino acids (aa) in length. It contains two CH/calponin homology domains (aa 99‑202 and 265‑369), two NLSs (aa 19‑26 and 38‑40), and a recently identified paxillin binding sequence (aa 248‑256). Multiple phosphorylation sites occur between Ser4 and Thr16 that are apparently utilized. PARVA is likely to exist as a dimer. There are three isoform variants of PARVA. All three contain an alternate start site at Met41, with one also showing a 21 aa substitution for aa 239‑372, and a second also showing a 46 aa substitution for aa 134‑372, respectively. Over aa 46‑133, human and mouse PARVA share 95% aa identity.

Entrez Gene IDs
55742 (Human); 57342 (Mouse)
Alternate Names
Actopaxin; alphaParvin; alpha-Parvin; Calponin-like integrin-linked kinase-binding protein; CH-ILKBP; FLJ10793; FLJ12254; FLJ25106; FLJ46049; Matrix-remodeling-associated protein 2; matrix-remodelling associated 2; MXRA2; PARVA; parvin, alpha

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human alpha-Parvin Antibody

There are currently no reviews for this product. Be the first to review Human alpha-Parvin Antibody and earn rewards!

Have you used Human alpha-Parvin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review