alpha -Parvin (parvus [Latin for "small"]-A/ alpha ; also actopaxin and PARV-A) is a 53 kDa member of the parvin family, alpha ‑actinin superfamily of proteins. It is widely expressed, and participates in actin filament assembly that precedes cell migration. Human PARVA is 372 amino acids (aa) in length. It contains two CH/calponin homology domains (aa 99‑202 and 265‑369), two NLSs (aa 19‑26 and 38‑40), and a recently identified paxillin binding sequence (aa 248‑256). Multiple phosphorylation sites occur between Ser4 and Thr16 that are apparently utilized. PARVA is likely to exist as a dimer. There are three isoform variants of PARVA. All three contain an alternate start site at Met41, with one also showing a 21 aa substitution for aa 239‑372, and a second also showing a 46 aa substitution for aa 134‑372, respectively. Over aa 46‑133, human and mouse PARVA share 95% aa identity.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Val46-Phe133
Accession # Q9NVD7
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human alpha-Parvin Antibody
Detection of Human alpha -Parvin by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Parvin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5556) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Parvin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Applications for Human alpha-Parvin Antibody
Western Blot
Sample: HUVEC human umbilical vein endothelial cells and human tonsil tissue
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: alpha-Parvin
Alternate Names
Gene Symbol
UniProt
Additional alpha-Parvin Products
Product Documents for Human alpha-Parvin Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human alpha-Parvin Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars