Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all alpha-Parvin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human alpha -Parvin
Val46-Phe133
Accession # Q9NVD7

Specificity

Detects human alpha -Parvin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human beta -Parvin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human alpha-Parvin Antibody

Detection of Human a-Parvin antibody by Western Blot.

Detection of Human alpha -Parvin by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Parvin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5556) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Parvin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human alpha-Parvin Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and human tonsil tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-Parvin

alpha -Parvin (parvus [Latin for "small"]-A/ alpha ; also actopaxin and PARV-A) is a 53 kDa member of the parvin family, alpha ‑actinin superfamily of proteins. It is widely expressed, and participates in actin filament assembly that precedes cell migration. Human PARVA is 372 amino acids (aa) in length. It contains two CH/calponin homology domains (aa 99‑202 and 265‑369), two NLSs (aa 19‑26 and 38‑40), and a recently identified paxillin binding sequence (aa 248‑256). Multiple phosphorylation sites occur between Ser4 and Thr16 that are apparently utilized. PARVA is likely to exist as a dimer. There are three isoform variants of PARVA. All three contain an alternate start site at Met41, with one also showing a 21 aa substitution for aa 239‑372, and a second also showing a 46 aa substitution for aa 134‑372, respectively. Over aa 46‑133, human and mouse PARVA share 95% aa identity.

Alternate Names

Actopaxin, alphaParvin, CH-ILKBP, MXRA2, PARVA

Entrez Gene IDs

55742 (Human); 57342 (Mouse)

Gene Symbol

PARVA

UniProt

Q9NVD7

Additional alpha-Parvin Products

Product Documents for Human alpha-Parvin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human alpha-Parvin Antibody

For research use only

Related Research Areas

Integrins and Associated Molecules

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