Aminopeptidase PILS/ARTS1 is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum). ARTS1 is widely expressed and releases an N-terminal amino acid (Leu). The amino acid sequence of mouse ARTS1 is 94%, 84%, 80 and 70% identical to that of rat, human, canine, and chicken.
Human Aminopeptidase PILS/ARTS1 Antibody (325813)
R&D Systems | Catalog # MAB23341
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 325813
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase PILS/ARTS1
Thr25-Ile929
Accession # Q9NZ08
Thr25-Ile929
Accession # Q9NZ08
Specificity
Detects human Aminopeptidase PILS/ARTS1 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Aminopeptidase PILS/ARTS1 Antibody (325813)
Aminopeptidase PILS/ARTS1 in HeLa Human Cell Line.
Aminopeptidase PILS/ARTS1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB23341) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Aminopeptidase PILS/ARTS1 Antibody (325813)
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Aminopeptidase PILS/ARTS1
Long Name
Aminopeptidase Puromycin-insensitive Leucyl-specific
Alternate Names
A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP
Gene Symbol
ERAP1
UniProt
Additional Aminopeptidase PILS/ARTS1 Products
Product Documents for Human Aminopeptidase PILS/ARTS1 Antibody (325813)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Aminopeptidase PILS/ARTS1 Antibody (325813)
For research use only
Related Research Areas
Customer Reviews for Human Aminopeptidase PILS/ARTS1 Antibody (325813)
There are currently no reviews for this product. Be the first to review Human Aminopeptidase PILS/ARTS1 Antibody (325813) and earn rewards!
Have you used Human Aminopeptidase PILS/ARTS1 Antibody (325813)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...