Human Amyloid beta (aa1-42) Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DAB142
Control Products Available
Human Amyloid beta aa 1-42 ELISA Standard Curve
2 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews

Human Amyloid beta (aa1-42) Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity
4.73 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)
Specificity
Natural human Amyloid beta (aa1-42).
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Amyloid beta (aa1-42) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-42) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-42) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta
(aa1-42) showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-42).

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 46.3 124 264 48.9 136 274
Standard Deviation 2.46 4.4 9.38 3.48 9.02 16.1
CV% 5.3 3.5 3.6 7.1 6.6 5.9

Recovery

The recovery of human Amyloid beta (aa1-42) spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 82-114
Cell Lysis Buffer (n=2) 92 90-94
Cerebrospinal Fluid (n=4) 107 95-120

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta (aa1-42) were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.
Human Amyloid beta aa 1-42 ELISA Linearity

Data Examples

Human Amyloid beta aa 1-42 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Amyloid beta

Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.

Entrez Gene IDs:
351 (Human); 11820 (Mouse)
Alternate Names:
Abeta; Amyloid beta; beta-Amyloid Peptide
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Amyloid beta (aa1-42) Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Urolithin A attenuates memory impairment and neuroinflammation in APP/PS1 mice
    Authors: Z Gong, J Huang, B Xu, Z Ou, L Zhang, X Lin, X Ye, X Kong, D Long, X Sun, X He, L Xu, Q Li, A Xuan
    J Neuroinflammation, 2019;16(1):62.
    Species: Mouse
    Sample Types: Tissue Homogenates
  2. The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro
    Authors: P Liu, X Chen, H Zhou, L Wang, Z Zhang, X Ren, F Zhu, Y Guo, X Huang, J Liu, PS Spencer, X Yang
    Oxid Med Cell Longev, 2018;2018(0):2025914.
    Species: Mouse
    Sample Types: Cell Lysates
  3. MPTAC Determines APP Fragmentation via Sensing Sulfur Amino Acid Catabolism
    Authors: T Suganuma, SK Swanson, M Gogol, TJ Garrett, J Conkright-, L Florens, MP Washburn, JL Workman
    Cell Rep, 2018;24(6):1585-1596.
    Species: Human
    Sample Types: Cell Lysates
  4. The neuroprotective effect of deep brain stimulation at nucleus basalis of Meynert in transgenic mice with Alzheimer's disease
    Authors: C Huang, H Chu, Y Ma, Z Zhou, C Dai, X Huang, L Fang, Q Ao, D Huang
    Brain Stimul, 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
  5. HDAC3 negatively regulates spatial memory in a mouse model of Alzheimer's disease
    Authors: X Zhu, S Wang, L Yu, J Jin, X Ye, Y Liu, Y Xu
    Aging Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. Folic acid supplementation improves cognitive function by reducing the levels of peripheral inflammatory cytokines in elderly Chinese subjects with MCI
    Sci Rep, 2016;6(0):37486.
    Species: Human
    Sample Types: Serum

FAQs

  1. Which form of A-beta (1-42) - monomer or oligomer - is detectable in Catalog # DAB142 (Human Amyloid beta (aa1-42) Quantikine ELISA kit)?

    • The Human Amyloid beta (aa1-42) Quantikine ELISA kit recognizes both monomers and oligomers of Human Amyloid beta (aa1-42). The standard in the kit is synthetic human Amyloid beta peptide that cannot oligomerize.

View all ELISA FAQs

ELISA Controls

Quantikine Colorimetric Sandwich ELISAs

Supplemental ELISA Products

Reviews for Human Amyloid beta (aa1-42) Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Human Amyloid beta (aa1-42) Quantikine ELISA Kit and earn rewards!

Have you used Human Amyloid beta (aa1-42) Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review