Human Amyloid beta (aa1-42) Quantikine ELISA Kit Summary
(aa1-42) showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-42).
Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human Amyloid beta (aa1-42) spiked to levels throughout the range of the assay in various matrices was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||97||82-114|
|Cell Lysis Buffer (n=2)||92||90-94|
|Cerebrospinal Fluid (n=4)||107||95-120|
Preparation and Storage
Background: Amyloid beta
Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.
Which form of A-beta (1-42) - monomer or oligomer - is detectable in Catalog # DAB142 (Human Amyloid beta (aa1-42) Quantikine ELISA kit)?
The Human Amyloid beta (aa1-42) Quantikine ELISA kit recognizes both monomers and oligomers of Human Amyloid beta (aa1-42). The standard in the kit is synthetic human Amyloid beta peptide that cannot oligomerize.
Supplemental ELISA Products
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