Human Amyloid beta (aa1-42) Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DAB142
Control Products Available
Human Amyload beta ELISA Standard Curve
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Product Details
Citations (22)
FAQs
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Human Amyloid beta (aa1-42) Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity
4.73 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)
Specificity
Natural human Amyloid beta (aa1-42).
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Amyloid beta (aa1-42) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-42) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-42) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta
(aa1-42) showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-42).

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 46.3 124 264 48.9 136 274
Standard Deviation 2.46 4.4 9.38 3.48 9.02 16.1
CV% 5.3 3.5 3.6 7.1 6.6 5.9

Recovery

The recovery of human Amyloid beta (aa1-42) spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 82-114
Cell Lysis Buffer (n=2) 92 90-94
Cerebrospinal Fluid (n=4) 107 95-120

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta (aa1-42) were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.
Human Amyload beta ELISA Linearity

Scientific Data

Human Amyload beta ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Amyloid beta

Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.

Entrez Gene IDs:
351 (Human); 11820 (Mouse)
Alternate Names:
Abeta; Amyloid beta; beta-Amyloid Peptide
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Amyloid beta (aa1-42) Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. An in vitro workflow of neuron-laden agarose-laminin hydrogel for studying small molecule-induced amyloidogenic condition
    Authors: P Namchaiw, P Bunreangsr, P Eiamcharoe, S Eiamboonse, R P Poo-Arpo
    PLoS ONE, 2022;17(8):e0273458.
    Species: Human
    Sample Types: Cell Lysates
  2. Potential Therapeutic Activity of Berberine in Thyroid-Associated Ophthalmopathy: Inhibitory Effects on Tissue Remodeling in Orbital Fibroblasts
    Authors: J Diao, X Chen, P Mou, X Ma, R Wei
    Investigative Ophthalmology & Visual Science, 2022;63(10):6.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. High frequency repetitive transcranial magnetic stimulation alleviates cognitive deficits in 3xTg-AD mice by modulating the PI3K/Akt/GLT-1 axis
    Authors: H Cao, C Zuo, Z Gu, Y Huang, Y Yang, L Zhu, Y Jiang, F Wang
    Redox Biology, 2022;54(0):102354.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Blood circulating miR-28-5p and let-7d-5p associate with premature ageing in Down syndrome
    Authors: C Morsiani, MG Bacalini, S Collura, M Moreno-Vil, N Breusing, A Bürkle, T Grune, C Franceschi, M De Eguileo, M Capri
    Mechanisms of Ageing and Development, 2022;206(0):111691.
    Species: Human
    Sample Types: Plasma
  5. Cognitive enhancement and neuroprotective effects of OABL, a sesquiterpene lactone in 5xFAD Alzheimer's disease mice model
    Authors: JJ Tang, LF Huang, JL Deng, YM Wang, C Guo, XN Peng, Z Liu, JM Gao
    Redox Biology, 2022;50(0):102229.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
  6. Recurrent moderate hypoglycemia accelerates the progression of cognitive deficits through impairment of TRPC6/GLUT3 pathway in diabetic APP/PS1 mice
    Authors: C He, Q Li, Y Cui, P Gao, W Shu, Q Zhou, L Wang, L Li, Z Lu, Y Zhao, H Ma, X Chen, H Jia, H Zheng, G Yang, D Liu, M Tepel, Z Zhu
    JCI Insight, 2022;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Development of a Low-Molecular-Weight Abeta42 Detection System Using a Enzyme-Linked Peptide Assay
    Authors: SH Kim, EH Lee, HJ Kim, AR Kim, YE Kim, JH Lee, MY Yoon, SH Koh
    Biomolecules, 2021;11(12):.
    Species: Human
    Sample Types: CSF
  8. CK1delta-Derived Peptides as Novel Tools Inhibiting the Interactions between CK1delta and APP695 to Modulate the Pathogenic Metabolism of APP
    Authors: A Roth, F Gärtner, K Mayer, J Beyrle, I König, U Knippschil, J Bischof
    International Journal of Molecular Sciences, 2021;22(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. CCR5 antagonist reduces HIV-induced amyloidogenesis, tau pathology, neurodegeneration, and blood-brain barrier alterations in HIV-infected hu-PBL-NSG mice
    Authors: B Bhargavan, SM Woollard, JE McMillan, GD Kanmogne
    Molecular Neurodegeneration, 2021;16(1):78.
    Species: Human
    Sample Types: Cell Lysates
  10. Microglial Calhm2 regulates neuroinflammation and contributes to Alzheimer's disease pathology
    Authors: J Cheng, Y Dong, J Ma, R Pan, Y Liao, X Kong, X Li, S Li, P Chen, L Wang, Y Yu, Z Yuan
    Science Advances, 2021;7(35):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Regulation of beta-amyloid production in neurons by astrocyte-derived cholesterol
    Authors: H Wang, JA Kulas, C Wang, DM Holtzman, HA Ferris, SB Hansen
    Proceedings of the National Academy of Sciences of the United States of America, 2021;118(33):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Serum Amyloid Beta42 Is Not Eliminated by the Cirrhotic Liver: A Pilot Study
    Authors: R Wiest, TS Weiss, L Danielyan, C Buechler
    Journal of Clinical Medicine, 2021;10(12):.
    Species: Human
    Sample Types: Serum
  13. Thymosin &beta4 reverses phenotypic polarization of glial cells and cognitive impairment via negative regulation of NF-&kappaB signaling axis in APP/PS1 mice
    Authors: M Wang, LR Feng, ZL Li, KG Ma, KW Chang, XL Chen, PB Yang, SF Ji, YB Ma, H Han, JB Ruganzua, WN Yang, YH Qian
    Journal of Neuroinflammation, 2021;18(1):146.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. APOE2 mitigates disease-related phenotypes in an isogenic hiPSC-based model of Alzheimer's disease
    Authors: N Brookhouse, S Raman, C Frisch, G Srinivasan, DA Brafman
    Molecular Psychiatry, 2021;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. The "develOpment of metabolic and functional markers of Dementia IN Older people" (ODINO) Study: Rationale, Design and Methods
    Authors: A Picca, D Ronconi, HJ Coelho-Jun, R Calvani, F Marini, A Biancolill, J Gervasoni, A Primiano, C Pais, E Meloni, D Fusco, MR Lo Monaco, R Bernabei, MC Cipriani, E Marzetti, R Liperoti
    J Pers Med, 2020;10(2):.
    Species: Human
    Sample Types: Serum
  16. Amyloid Beta Peptide (A&beta1-42) Reverses the Cholinergic Control of Monocytic IL-1&beta Release
    Authors: K Richter, R Ogiemwonyi, S Wilker, AI Chaveiro, A Agné, M Hecker, M Reichert, AL Amati, KD Schlüter, I Manzini, G Schmalzing, JM McIntosh, W Padberg, V Grau, A Hecker
    J Clin Med, 2020;9(9):.
    Species: Human
    Sample Types: Plasma
  17. Urolithin A attenuates memory impairment and neuroinflammation in APP/PS1 mice
    Authors: Z Gong, J Huang, B Xu, Z Ou, L Zhang, X Lin, X Ye, X Kong, D Long, X Sun, X He, L Xu, Q Li, A Xuan
    J Neuroinflammation, 2019;16(1):62.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. MPTAC Determines APP Fragmentation via Sensing Sulfur Amino Acid Catabolism
    Authors: T Suganuma, SK Swanson, M Gogol, TJ Garrett, J Conkright-, L Florens, MP Washburn, JL Workman
    Cell Rep, 2018;24(6):1585-1596.
    Species: Human
    Sample Types: Cell Lysates
  19. The neuroprotective effect of deep brain stimulation at nucleus basalis of Meynert in transgenic mice with Alzheimer's disease
    Authors: C Huang, H Chu, Y Ma, Z Zhou, C Dai, X Huang, L Fang, Q Ao, D Huang
    Brain Stimul, 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
  20. The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro
    Authors: P Liu, X Chen, H Zhou, L Wang, Z Zhang, X Ren, F Zhu, Y Guo, X Huang, J Liu, PS Spencer, X Yang
    Oxid Med Cell Longev, 2018;2018(0):2025914.
    Species: Mouse
    Sample Types: Cell Lysates
  21. HDAC3 negatively regulates spatial memory in a mouse model of Alzheimer's disease
    Authors: X Zhu, S Wang, L Yu, J Jin, X Ye, Y Liu, Y Xu
    Aging Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. Folic acid supplementation improves cognitive function by reducing the levels of peripheral inflammatory cytokines in elderly Chinese subjects with MCI
    Sci Rep, 2016;6(0):37486.
    Species: Human
    Sample Types: Serum

FAQs

  1. Which form of A-beta (1-42) - monomer or oligomer - is detectable in Catalog # DAB142 (Human Amyloid beta (aa1-42) Quantikine ELISA kit)?

    • The Human Amyloid beta (aa1-42) Quantikine ELISA kit recognizes both monomers and oligomers of Human Amyloid beta (aa1-42). The standard in the kit is synthetic human Amyloid beta peptide that cannot oligomerize.

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