Human Angiopoietin-1 DuoSet ELISA

(16 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Sample Type & Volume Required
    100 µL
  • Range
    156.00 - 10,000 pg/mL
  • Sufficient Materials
    For fifteen 96-well plates*
  • Specificity
    Please see the product datasheet
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Ancillary Reagent Kit Available

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.


Product Features
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
Kit Content
  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiopoietin-1
Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.
  • Entrez Gene IDs:
    284 (Human); 11600 (Mouse);
  • Aliases:
    AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta.
    Authors: Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C
    PLoS Negl Trop Dis, 2015;9(6):e0003824.
    Species: Human
    Sample Type: Plasma
  2. Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
    Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
    PLoS ONE, 2015;10(8):e0134619.
    Species: Human
    Sample Type: Plasma
  3. Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha.
    Authors: Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J
    PLoS ONE, 2015;10(9):e0137612.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. The effect of nitrogen containing bisphosphonates, zoledronate and alendronate, on the production of pro-angiogenic factors by osteoblastic cells.
    Authors: Ishtiaq S, Edwards S, Sankaralingam A, Evans B, Elford C, Frost M, Fogelman I, Hampson G
    Cytokine, 2015;71(2):154-60.
    Species: Human
    Sample Type: Serum
  5. Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
    Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
    BMC Infect Dis, 2014;14(0):35.
  6. Increased endothelial inflammation, sTie-2 and arginase activity in umbilical cords obtained from gestational diabetic mothers.
    Authors: Giri H, Chandel S, Dwarakanath L, Sreekumar S, Dixit M
    PLoS ONE, 2013;8(12):e84546.
    Species: Human
    Sample Type: Serum
  7. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011;6(12):e28540.
    Species: Human
    Sample Type: Plasma
  8. Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
    Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
    PLoS ONE, 2010;5(12):e15291.
    Species: Human
    Sample Type: Plasma
  9. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010;24(10):3782-95.
    Species: Human
    Sample Type: Cell Culture Supernates
  10. Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy.
    Authors: Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN
    Placenta, 2010;31(6):545-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  11. Serum levels of angiogenic factors and their prognostic relevance in bladder cancer.
    Authors: Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H
    Pathol. Oncol. Res., 2009;15(2):193-201.
    Species: Human
    Sample Type: Serum
  12. Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia.
    Authors: Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H
    Am. J. Hypertens., 2009;22(8):891-5.
    Species: Human
    Sample Type: Plasma
  13. Role of vitamin D receptor in the antiproliferative effects of calcitriol in tumor-derived endothelial cells and tumor angiogenesis in vivo.
    Authors: Chung I, Han G, Seshadri M, Gillard BM, Yu WD, Foster BA, Trump DL, Johnson CS
    Cancer Res., 2009;69(3):967-75.
    Species: Mouse
    Sample Type: Cell Lysates
  14. Angiogenesis and lymphangiogenesis are downregulated in primary breast cancer.
    Authors: Boneberg EM, Legler DF, Hoefer MM, Ohlschlegel C, Steininger H, Fuzesi L, Beer GM, Dupont-Lampert V, Otto F, Senn HJ, Furstenberger G
    Br. J. Cancer, 2009;101(4):605-14.
    Species: Human
    Sample Type: Serum
  15. Intradermal delivery of plasmid VEGF(165) by electroporation promotes wound healing.
    Authors: Ferraro B, Cruz YL, Coppola D, Heller R
    Mol. Ther., 2009;17(4):651-7.
    Species: Rat
    Sample Type: Cell Culture Supernates
  16. Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients.
    Authors: van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB
    Thorax, 2008;63(10):903-9.
    Species: Human
    Sample Type: Plasma
Expand to show all 16 Citations
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