Human Angiopoietin-1 DuoSet ELISA
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Summary
Product Features
Kit Content
Other Reagents Required
Data Examples
Product Datasheets
Preparation and Storage
Background
Human Angiopoietin-1 DuoSet ELISA Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
156.0 - 10,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiopoietin-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
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Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiopoietin-1
Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.
Entrez Gene IDs:
284 (Human); 11600 (Mouse)
Alternate Names:
AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003
Citations for Human Angiopoietin-1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
Journal of ovarian research, 2022;15(1):87.
Species: Human
Sample Types: Cell Culture Supernates
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Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus
Authors: M Reijrink, J van Ark, CPH Lexis, LM Visser, ME Lodewijk, ICC van der Ho, CJ Zeebregts, H van Goor, SCA de Jager, G Pasterkamp, BHR Wolffenbut, JL Hillebrand
Cardiovascular Diabetology, 2022;21(1):72.
Species: Human
Sample Types: Plasma
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Depolymerization of fucoidan with endo-fucoidanase changes bioactivity in processes relevant for bone regeneration
Authors: J Ohmes, MD Mikkelsen, TT Nguyen, VHN Tran, S Meier, MS Nielsen, M Ding, A Seekamp, AS Meyer, S Fuchs
Carbohydrate polymers, 2022;286(0):119286.
Species: Human
Sample Types: Cell Culture Supernates
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Cholecalciferol supplementation and angiogenic markers in chronic kidney disease
Authors: J Kaur, K Kamboj, AK Yadav, P Kaur, V Kumar, V Jha
PLoS ONE, 2022;17(6):e0268946.
Species: Human
Sample Types: Serum
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Angiogenic and angiostatic factors present in the saliva of malaria patients
Authors: CE Lekpor, F Botchway, KA Kusi, AA Adjei, MD Wilson, JK Stiles, NO Wilson
Malaria Journal, 2022;21(1):220.
Species: Human
Sample Types: Plasma
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The Angiopoietin-Tie2 axis contributes to placental vascular disruption and adverse birth outcomes in malaria in pregnancy
Authors: V Tran, AM Weckman, VM Crowley, LS Cahill, K Zhong, A Cabrera, RE Elphinston, V Pearce, M Madanitsa, L Kalilani-P, V Mwapasa, C Khairallah, AL Conroy, FO Ter Kuile, JG Sled, KC Kain
EBioMedicine, 2021;73(0):103683.
Species: Mouse
Sample Types: Plasma
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ICU Admission Levels of Endothelial Biomarkers as Predictors of Mortality in Critically Ill COVID-19 Patients
Authors: AG Vassiliou, C Keskinidou, E Jahaj, P Gallos, I Dimopoulou, A Kotanidou, SE Orfanos
Cells, 2021;10(1):.
Species: Human
Sample Types: Plasma
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Angiopoietin 1 release from human neutrophils is independent from neutrophil extracellular traps (NETs)
Authors: E Charles, BL Dumont, S Bonneau, PE Neagoe, L Villeneuve, A Räkel, M White, MG Sirois
BMC immunology, 2021;22(1):51.
Species: Human
Sample Types: Cell Culture Supernates
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Analysis of Inflammatory Mediator Profiles in Sepsis Patients Reveals That Extracellular Histones Are Strongly Elevated in Nonsurvivors
Authors: T Eichhorn, I Linsberger, L Lauková, C Tripiscian, B Fendl, R Weiss, F König, G Valicek, G Miestinger, C Hörmann, V Weber
Mediators of Inflammation, 2021;2021(0):8395048.
Species: Human
Sample Types: Plasma
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Serum protein triplet TGF-&beta1, TIMP-1, and YKL-40 serve as diagnostic and prognostic profile for astrocytoma
Authors: R Urbanavi?i, R Zabitait?, A Kriš?iukai, VP Deltuva, D Skiriut?
Scientific Reports, 2021;11(1):13100.
Species: Human
Sample Types: Serum
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Significance of Serum Angiopoietin-2 in Patients with Hemorrhage in Adult-Onset Moyamoya Disease
Authors: J Yu, K Huang, J Pan, J Shen, R Zhan
Biomed Res Int, 2020;2020(0):8209313.
Species: Human
Sample Types: Serum
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Angiopoietins, Vascular Endothelial Growth Factors and Secretory Phospholipase A2 in Ischemic and Non-Ischemic Heart Failure
Authors: G Varricchi, S Loffredo, L Bencivenga, AL Ferrara, G Gambino, N Ferrara, A de Paulis, G Marone, G Rengo
J Clin Med, 2020;9(6):.
Species: Human
Sample Types: Plasma
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Dysregulation of angiopoietin-Tie-2 axis in ugandan children hospitalized with pneumonia
Authors: R Zhang, U Rai, NBM Ibrahim, Y Amazouz, J Soo, AL Conroy, S Namasopo, RO Opoka, R Bhargava, MT Hawkes
Cytokine, 2020;133(0):155175.
Species: Human
Sample Types: Plasma
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Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment
Authors: J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs
Mar Drugs, 2020;18(9):.
Species: Human
Sample Types: Cell Culture Supernates
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Association of Angiopoietin Dysregulation in Placental Malaria with Adverse Birth Outcomes
Authors: PP Singh, S Bhandari, RK Sharma, N Singh, PK Bharti
Dis. Markers, 2020;2020(0):6163487.
Species: Human
Sample Types: Plasma
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Progestin-induced heart and neural crest derivatives-expressed transcript 2 inhibits angiopoietin 2 via fibroblast growth factor 9 in human endometrial stromal cells
Authors: H Murata, T Tsuzuki, T Kido, M Kakita-Kob, N Kida, Y Hisamatsu, H Okada
Reprod Biol, 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Gamma-Tocotrienol Induces Apoptosis in Prostate Cancer Cells by Targeting the Ang-1/Tie-2 Signalling Pathway
Authors: KD Tang, J Liu, PJ Russell, JA Clements, MT Ling
Int J Mol Sci, 2019;20(5):.
Species: Human
Sample Types: Cell Culture Supernates
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Hepatic blood flow by perfusion computed tomography as an imaging biomarker for patients with gastric cancer
Authors: K Shuto, M Mori, C Kosugi, K Narushima, S Nakabayash, T Fujisiro, A Sato, K Hayano, H Shimizu, K Koda
Oncol Lett, 2019;17(3):3267-3276.
Species: Human
Sample Types: Serum
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Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study
Authors: JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin
BMC Pediatr, 2018;18(1):118.
Species: Human
Sample Types: Plasma
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Tie2 protects the vasculature against thrombus formation in systemic inflammation
Authors: SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh
J. Clin. Invest., 2018;0(0):.
Species: Mouse
Sample Types: Plasma
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Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
PLoS ONE, 2015;10(8):e0134619.
Species: Human
Sample Types: Plasma
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Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta.
Authors: Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C
PLoS Negl Trop Dis, 2015;9(6):e0003824.
Species: Human
Sample Types: Plasma
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Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha.
Authors: Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J
PLoS ONE, 2015;10(9):e0137612.
Species: Human
Sample Types: Cell Culture Supernates
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The effect of nitrogen containing bisphosphonates, zoledronate and alendronate, on the production of pro-angiogenic factors by osteoblastic cells.
Authors: Ishtiaq S, Edwards S, Sankaralingam A, Evans B, Elford C, Frost M, Fogelman I, Hampson G
Cytokine, 2015;71(2):154-60.
Species: Human
Sample Types: Serum
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Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
BMC Infect Dis, 2014;14(0):35.
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Increased endothelial inflammation, sTie-2 and arginase activity in umbilical cords obtained from gestational diabetic mothers.
Authors: Giri H, Chandel S, Dwarakanath L, Sreekumar S, Dixit M
PLoS ONE, 2013;8(12):e84546.
Species: Human
Sample Types: Serum
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Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
Authors: Conroy AL, Liles WC, Molyneux ME
PLoS ONE, 2011;6(12):e28540.
Species: Human
Sample Types: Plasma
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Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy.
Authors: Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN
Placenta, 2010;31(6):545-8.
Species: Human
Sample Types: Cell Culture Supernates
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Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
PLoS ONE, 2010;5(12):e15291.
Species: Human
Sample Types: Plasma
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Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
FASEB J., 2010;24(10):3782-95.
Species: Human
Sample Types: Cell Culture Supernates
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Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia.
Authors: Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H
Am. J. Hypertens., 2009;22(8):891-5.
Species: Human
Sample Types: Plasma
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Intradermal delivery of plasmid VEGF(165) by electroporation promotes wound healing.
Authors: Ferraro B, Cruz YL, Coppola D, Heller R
Mol. Ther., 2009;17(4):651-7.
Species: Rat
Sample Types: Cell Culture Supernates
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Angiogenesis and lymphangiogenesis are downregulated in primary breast cancer.
Authors: Boneberg EM, Legler DF, Hoefer MM, Ohlschlegel C, Steininger H, Fuzesi L, Beer GM, Dupont-Lampert V, Otto F, Senn HJ, Furstenberger G
Br. J. Cancer, 2009;101(4):605-14.
Species: Human
Sample Types: Serum
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Serum levels of angiogenic factors and their prognostic relevance in bladder cancer.
Authors: Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H
Pathol. Oncol. Res., 2009;15(2):193-201.
Species: Human
Sample Types: Serum
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Role of vitamin D receptor in the antiproliferative effects of calcitriol in tumor-derived endothelial cells and tumor angiogenesis in vivo.
Authors: Chung I, Han G, Seshadri M, Gillard BM, Yu WD, Foster BA, Trump DL, Johnson CS
Cancer Res., 2009;69(3):967-75.
Species: Mouse
Sample Types: Cell Lysates
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Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients.
Authors: van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB
Thorax, 2008;63(10):903-9.
Species: Human
Sample Types: Plasma
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Human Angiopoietin-1 DuoSet ELISA
By Anonymous
on 10/07/2019
Sample Tested: Recombinant protein
