Human Angiopoietin-1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
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Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: Angiopoietin-1
Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.
Citations for Human Angiopoietin-1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
Journal of ovarian research, 2022;15(1):87.
Species: Human
Sample Types: Cell Culture Supernates
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Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus
Authors: M Reijrink, J van Ark, CPH Lexis, LM Visser, ME Lodewijk, ICC van der Ho, CJ Zeebregts, H van Goor, SCA de Jager, G Pasterkamp, BHR Wolffenbut, JL Hillebrand
Cardiovascular Diabetology, 2022;21(1):72.
Species: Human
Sample Types: Plasma
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Depolymerization of fucoidan with endo-fucoidanase changes bioactivity in processes relevant for bone regeneration
Authors: J Ohmes, MD Mikkelsen, TT Nguyen, VHN Tran, S Meier, MS Nielsen, M Ding, A Seekamp, AS Meyer, S Fuchs
Carbohydrate polymers, 2022;286(0):119286.
Species: Human
Sample Types: Cell Culture Supernates
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Cholecalciferol supplementation and angiogenic markers in chronic kidney disease
Authors: J Kaur, K Kamboj, AK Yadav, P Kaur, V Kumar, V Jha
PLoS ONE, 2022;17(6):e0268946.
Species: Human
Sample Types: Serum
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Angiogenic and angiostatic factors present in the saliva of malaria patients
Authors: CE Lekpor, F Botchway, KA Kusi, AA Adjei, MD Wilson, JK Stiles, NO Wilson
Malaria Journal, 2022;21(1):220.
Species: Human
Sample Types: Plasma
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The Angiopoietin-Tie2 axis contributes to placental vascular disruption and adverse birth outcomes in malaria in pregnancy
Authors: V Tran, AM Weckman, VM Crowley, LS Cahill, K Zhong, A Cabrera, RE Elphinston, V Pearce, M Madanitsa, L Kalilani-P, V Mwapasa, C Khairallah, AL Conroy, FO Ter Kuile, JG Sled, KC Kain
EBioMedicine, 2021;73(0):103683.
Species: Mouse
Sample Types: Plasma
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ICU Admission Levels of Endothelial Biomarkers as Predictors of Mortality in Critically Ill COVID-19 Patients
Authors: AG Vassiliou, C Keskinidou, E Jahaj, P Gallos, I Dimopoulou, A Kotanidou, SE Orfanos
Cells, 2021;10(1):.
Species: Human
Sample Types: Plasma
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Angiopoietin 1 release from human neutrophils is independent from neutrophil extracellular traps (NETs)
Authors: E Charles, BL Dumont, S Bonneau, PE Neagoe, L Villeneuve, A Räkel, M White, MG Sirois
BMC immunology, 2021;22(1):51.
Species: Human
Sample Types: Cell Culture Supernates
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Analysis of Inflammatory Mediator Profiles in Sepsis Patients Reveals That Extracellular Histones Are Strongly Elevated in Nonsurvivors
Authors: T Eichhorn, I Linsberger, L Lauková, C Tripiscian, B Fendl, R Weiss, F König, G Valicek, G Miestinger, C Hörmann, V Weber
Mediators of Inflammation, 2021;2021(0):8395048.
Species: Human
Sample Types: Plasma
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Serum protein triplet TGF-&beta1, TIMP-1, and YKL-40 serve as diagnostic and prognostic profile for astrocytoma
Authors: R Urbanavi?i, R Zabitait?, A Kriš?iukai, VP Deltuva, D Skiriut?
Scientific Reports, 2021;11(1):13100.
Species: Human
Sample Types: Serum
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Significance of Serum Angiopoietin-2 in Patients with Hemorrhage in Adult-Onset Moyamoya Disease
Authors: J Yu, K Huang, J Pan, J Shen, R Zhan
Biomed Res Int, 2020;2020(0):8209313.
Species: Human
Sample Types: Serum
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Angiopoietins, Vascular Endothelial Growth Factors and Secretory Phospholipase A2 in Ischemic and Non-Ischemic Heart Failure
Authors: G Varricchi, S Loffredo, L Bencivenga, AL Ferrara, G Gambino, N Ferrara, A de Paulis, G Marone, G Rengo
J Clin Med, 2020;9(6):.
Species: Human
Sample Types: Plasma
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Dysregulation of angiopoietin-Tie-2 axis in ugandan children hospitalized with pneumonia
Authors: R Zhang, U Rai, NBM Ibrahim, Y Amazouz, J Soo, AL Conroy, S Namasopo, RO Opoka, R Bhargava, MT Hawkes
Cytokine, 2020;133(0):155175.
Species: Human
Sample Types: Plasma
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Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment
Authors: J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs
Mar Drugs, 2020;18(9):.
Species: Human
Sample Types: Cell Culture Supernates
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Association of Angiopoietin Dysregulation in Placental Malaria with Adverse Birth Outcomes
Authors: PP Singh, S Bhandari, RK Sharma, N Singh, PK Bharti
Dis. Markers, 2020;2020(0):6163487.
Species: Human
Sample Types: Plasma
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Progestin-induced heart and neural crest derivatives-expressed transcript 2 inhibits angiopoietin 2 via fibroblast growth factor 9 in human endometrial stromal cells
Authors: H Murata, T Tsuzuki, T Kido, M Kakita-Kob, N Kida, Y Hisamatsu, H Okada
Reprod Biol, 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Gamma-Tocotrienol Induces Apoptosis in Prostate Cancer Cells by Targeting the Ang-1/Tie-2 Signalling Pathway
Authors: KD Tang, J Liu, PJ Russell, JA Clements, MT Ling
Int J Mol Sci, 2019;20(5):.
Species: Human
Sample Types: Cell Culture Supernates
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Hepatic blood flow by perfusion computed tomography as an imaging biomarker for patients with gastric cancer
Authors: K Shuto, M Mori, C Kosugi, K Narushima, S Nakabayash, T Fujisiro, A Sato, K Hayano, H Shimizu, K Koda
Oncol Lett, 2019;17(3):3267-3276.
Species: Human
Sample Types: Serum
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Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study
Authors: JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin
BMC Pediatr, 2018;18(1):118.
Species: Human
Sample Types: Plasma
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Tie2 protects the vasculature against thrombus formation in systemic inflammation
Authors: SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh
J. Clin. Invest., 2018;0(0):.
Species: Mouse
Sample Types: Plasma
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Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
PLoS ONE, 2015;10(8):e0134619.
Species: Human
Sample Types: Plasma
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Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta.
Authors: Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C
PLoS Negl Trop Dis, 2015;9(6):e0003824.
Species: Human
Sample Types: Plasma
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Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha.
Authors: Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J
PLoS ONE, 2015;10(9):e0137612.
Species: Human
Sample Types: Cell Culture Supernates
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The effect of nitrogen containing bisphosphonates, zoledronate and alendronate, on the production of pro-angiogenic factors by osteoblastic cells.
Authors: Ishtiaq S, Edwards S, Sankaralingam A, Evans B, Elford C, Frost M, Fogelman I, Hampson G
Cytokine, 2015;71(2):154-60.
Species: Human
Sample Types: Serum
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Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
BMC Infect Dis, 2014;14(0):35.
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Increased endothelial inflammation, sTie-2 and arginase activity in umbilical cords obtained from gestational diabetic mothers.
Authors: Giri H, Chandel S, Dwarakanath L, Sreekumar S, Dixit M
PLoS ONE, 2013;8(12):e84546.
Species: Human
Sample Types: Serum
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Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
Authors: Conroy AL, Liles WC, Molyneux ME
PLoS ONE, 2011;6(12):e28540.
Species: Human
Sample Types: Plasma
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Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy.
Authors: Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN
Placenta, 2010;31(6):545-8.
Species: Human
Sample Types: Cell Culture Supernates
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Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
PLoS ONE, 2010;5(12):e15291.
Species: Human
Sample Types: Plasma
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Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
FASEB J., 2010;24(10):3782-95.
Species: Human
Sample Types: Cell Culture Supernates
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Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia.
Authors: Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H
Am. J. Hypertens., 2009;22(8):891-5.
Species: Human
Sample Types: Plasma
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Intradermal delivery of plasmid VEGF(165) by electroporation promotes wound healing.
Authors: Ferraro B, Cruz YL, Coppola D, Heller R
Mol. Ther., 2009;17(4):651-7.
Species: Rat
Sample Types: Cell Culture Supernates
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Angiogenesis and lymphangiogenesis are downregulated in primary breast cancer.
Authors: Boneberg EM, Legler DF, Hoefer MM, Ohlschlegel C, Steininger H, Fuzesi L, Beer GM, Dupont-Lampert V, Otto F, Senn HJ, Furstenberger G
Br. J. Cancer, 2009;101(4):605-14.
Species: Human
Sample Types: Serum
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Serum levels of angiogenic factors and their prognostic relevance in bladder cancer.
Authors: Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H
Pathol. Oncol. Res., 2009;15(2):193-201.
Species: Human
Sample Types: Serum
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Role of vitamin D receptor in the antiproliferative effects of calcitriol in tumor-derived endothelial cells and tumor angiogenesis in vivo.
Authors: Chung I, Han G, Seshadri M, Gillard BM, Yu WD, Foster BA, Trump DL, Johnson CS
Cancer Res., 2009;69(3):967-75.
Species: Mouse
Sample Types: Cell Lysates
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Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients.
Authors: van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB
Thorax, 2008;63(10):903-9.
Species: Human
Sample Types: Plasma
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