Human Angiopoietin-1 Luminex Performance Assay

LAN923 has been discontinued and is replaced by LANM923.
(1 citations)   
  • Assay Type
    Bead-based multiplex assay for the Luminex® platform
  • Format
    96-well microplate and antibody-coated beads*
  • Analytes Detected
    Please see table below.
  • Performance Validation
    For standard curves, intra-assay and inter-assay precision, recovery, and linearity data for the different analyte-specific beads, please see product datasheets for corresponding individual Bead Sets (below).

 

Product Features
  • Simultaneously detect multiple analytes
  • User selects specific analytes of interest
  • Requires small sample size
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific

 

Base Kit Contents
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent(s)
  • Streptavidin-PE
  • Wash Buffer Concentrate
  • Mixing Bottles
  • Filter-bottom Microplate
  • Foil Plate Sealers

 

Bead Set Contents
  • Antibody-coated beads
  • Biotinylated detection antibody

 

Information for Compatiable Analytes

For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.

Analyte Catalog
Number
Sensitivity
(pg/mL)
High Standard Value*
(pg/mL)
Microparticle
Region
Angiogenin LAN265 2.7 10,400 5
Angiopoietin-1 LAN923 51.1 149,000 22
Endostatin LAN1098 31.9 90,500 18
FGF acidic LAN232 6.7 15,800 33
FGF basic LUH233 4.9 2,200 54
PDGF-AA LAN221 1 7,350 51
PDGF-BB LAN220 1 21,800 37
PlGF LAN264 2 11,300 34
Thrombospondin-2 LAN1635 6.7 230,000 59
VEGF LUH293 1.8 2,400 52
VEGF-D LAN622 19.1 87,300 66
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

 

Validated Samples and Recommended Starting Dilution

Analyte Cell Culture Supernates Serum EDTA Plasma Platelet-poor EDTA Plasma Heparin Plasma Platelet-poor Heparin Plasma Urine Human Milk
Angiogenin 1:5 1:5 1:50 1:50 1:50 1:50 1:5 1:20
Angiopoietin-1* 1:5 1:5 N/R 1:5 N/R 1:5 1:5 1:5
Endostatin 1:5 1:5 1:5 1:5 1:5 1:5 1:5 1:5
FGF acidic 1:5 1:5 1:5 1:5 1:5 1:5 1:5 1:5
FGF basic 1:5 1:5 1:5 1:5 N/R N/R 1:5 1:5
PDGF-AA* 1:5 1:5 N/R 1:5 N/R 1:5 1:5 1:5
PDGF-BB* 1:5 1:5 N/R 1:5 N/R 1:5 1:5 1:5
PlGF 1:5 1:5 1:5 1:5 1:5 1:5 1:5 1:5
Thrombospondin-2 1:5 1:5 1:5 1:5 1:5 1:5 1:5 1:5
VEGF 1:5 1:5 1:5 1:5 1:5 1:5 1:5 1:5
VEGF-D 1:5 1:5 1:5 1:5 1:5 1:5 1:5 1:5
N/R=This sample type is not recommended for use in this assay
*Angiopoietin-1, PDGF-AA, and PDGF-BB are present in platelet granules and are released upon platelet activation. Therefore, to measure circulating levels of these factors, platelet-free plasma should be collected for measurement.

 

More Information about Bead-Based Multiple Kits for the Luminex Platform

Luminex High Performance Assays are designed for use with the Luminex 100™, Luminex 200™, or Bio-Rad® Bio-Plex® dual laser, flow-based sorting and detection analyzers manufactured by Luminex Corporation.

Analyte-specific antibodies are pre-coated onto color-coded beads. Beads, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the beads are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. The other laser determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound.

 

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

Trademarks and registered trademarks are the property of their respective company.

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Role of serum levels of angiogenic cytokines in assessment of angiogenesis after stem cell therapy of diabetic patients with critical limb ischemia.
    Authors: Dubsky M, Jirkovska A, Bem R, Fejfarova V, Varga M, Kolesar L, Pagacova L, Sykova E, Jude E
    Cell Transplant, 2014;23(12):1517-23.
    Species: Human
    Sample Type: Serum

Have you used Human Angiopoietin-1 Luminex Performance Assay?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

LAN923 has been replaced by
LANM923

Contact Information

R&D Systems Guarantee

Feedback

Customer Information