For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.
|High Standard Value*
|*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.|
|Analyte||Cell Culture Supernates||Serum||EDTA Plasma||Platelet-poor EDTA Plasma||Heparin Plasma||Platelet-poor Heparin Plasma||Urine||Human Milk|
|N/R=This sample type is not recommended for use in this assay
*Angiopoietin-1, PDGF-AA, and PDGF-BB are present in platelet granules and are released upon platelet activation. Therefore, to measure circulating levels of these factors, platelet-free plasma should be collected for measurement.
Luminex High Performance Assays are designed for use with the Luminex 100™, Luminex 200™, or Bio-Rad® Bio-Plex® dual laser, flow-based sorting and detection analyzers manufactured by Luminex Corporation.
Analyte-specific antibodies are pre-coated onto color-coded beads. Beads, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the beads are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. The other laser determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound.
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
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