Human Angiopoietin-like Protein 3/ANGPTL3 Antibody (388610)

R&D Systems | Catalog # MAB38292

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 388610
View all Angiopoietin-like Protein 3/ANGPTL3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Angiopoietin‑like Protein 3/ANGPTL3
Ile19-Glu460
Accession # Q9Y5C1

Specificity

Detects human Angiopoietin‑like Protein 3/ANGPTL3 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody (388610)

Detection of Human Angiopoietin-like Protein 3/ANGPTL3 antibody by Western Blot.

Detection of Human Angiopoietin‑like Protein 3/ANGPTL3 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line (negative control), HepG2 human hepatocellular carcinoma cell line, and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Angiopoietin-like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB38292) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-like Protein 3/ANGPTL3 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody (388610)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that belongs to the angiopoietin family. It is expressed in the liver and circulates as 70 kDa, 50 kDa, and 32 kDa species. These include full length ANGPTL3 as well as the proteolytically separated N- and C-terminal fragments which contain a coiled coil domain and fibrinogen-like domain, respectively. ANGPTL3 promotes an increase in circulating triglyceride levels by directly inhibiting lipoprotein lipase. Mature human ANGPTL3 shares 77% amino acid sequence identity with mouse ANGPTL3.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Gene Symbol

ANGPTL3

UniProt

Q9Y5C1 (Human)

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody (388610)

Certificate of Analysis

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Product Specific Notices for Human Angiopoietin-like Protein 3/ANGPTL3 Antibody (388610)

For research use only

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