Human Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody

Catalog #: BAF3829
1 Citation Datasheet / COA / SDS

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Angiopoietin-like 3 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross‑reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, rhAngiopoietin-4, rhANGPTL4, recombinant mouse (rm) Angiopoietin-3, rmANGPTL3 and rmANGPTL4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Angiopoietin-like 3 (R&D Systems, Catalog # 3829-AN)
Ser17-Glu460
Accession # Q9Y5C1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Angiopoietin-like 3 (Catalog # 3829-AN)

Human Angiopoietin-like Protein 3/ANGPTL3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human Angiopoietin-like 3 Antibody (Catalog # MAB38291)

Standard: Recombinant Human Angiopoietin-like 3 Protein, CF (Catalog # 3829-AN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins. Human ANGPTL3 contains an N‑terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C‑terminal fibrinogen-like domain that binds integrin alpha _v beta ₃. In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N‑terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C‑terminal fragment that can induce angiogenesis. Full length and cleaved ANGPTL3 circulate in plasma. Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively.

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Alternate Names

AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1