Recombinant Human Angiopoietin-like 3 Protein, CF

Catalog # Availability Size / Price Qty
3829-AN-050
Product Details
Citations (1)
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Recombinant Human Angiopoietin-like 3 Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED50 for this effect is 40-200 ng/mL in the presence of a cross-linking Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770. The IC50 value under conditions in which Recombinant Human Lipoprotein Lipase/LPL Protein, CF (Catalog # 9888-LL) and p-nitrophenyl butyrate are present in 0.1 M sodium phosphate, 0.15 M NaCl, 0.5% Triton® X-100, pH 7.2, is <15 µg/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Angiopoietin-like Protein 3/ANGPTL3 protein
Ser17-Glu460, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Ser17
Structure / Form
Oligomer
Predicted Molecular Mass
53.2 kDa
SDS-PAGE
54-59 kDa and 28-30 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3829-AN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and CHAPS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Angiopoietin-like Protein 3/ANGPTL3

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins (1-3). Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do proteolytically separated N- and C-terminal fragments containing the coiled-coil domain and fibrinogen-like domains (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids (8, 9). This activity requires a putative heparin-binding motif which is N-terminal to the coiled-coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6-8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (10). ANGPTL3 expression in vivo is up-regulated by LXR agonists and down-regulated by insulin, leptin, and agonists of TR beta or PPAR beta (11-14). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice (7, 8, 12). ANGPTL3 does not bind Tie1 or Tie2, but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (15). ANGPTL3, secreted by fetal liver, also promotes the expansion of hematopoietic stem cells (16). Mature human ANGPTL3 shares 24%-30% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 5, 6, and 7. It shares 77% aa sequence identity with mouse ANGPTL3.

References
  1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
  2. Oike, Y. et al. (2004) Int. J. Hematol. 80:21.
  3. Kersten, S. (2005) Biochem. Soc. Trans. 33:1059.
  4. Conklin, D. et al. (1999) Genomics 62:477.
  5. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  6. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
  7. Koishi, R. et al. (2002) Nat. Genet. 30:151.
  8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
  9. Shimamura, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:366.
  10. Koster, A. et al. (2005) Endocrinology 146:4943.
  11. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
  12. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
  13. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
  14. Matsusue, K. et al. (2006) Mol. Cell Endocrinol. 256:23.
  15. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
  16. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
Entrez Gene IDs
27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names
AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2

Citation for Recombinant Human Angiopoietin-like 3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Lipoprotein lipase activity and interactions studied in human plasma by isothermal titration calorimetry
    Authors: Aivar Lookene
    J. Lipid Res., 2016;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Bioassay

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