Human Angiopoietin-like Protein 3/ANGPTL3 Antibody

Human Angiopoietin-like Protein 3 / ANGPTL3 ELISA Standard Curve

Recombinant Human Angiopoietin‑like Protein 3/ANGPTL3 (Catalog # 3829-AN) was serially diluted and captured by Rat Anti-Human Angiopoietin‑like Protein 3/ANGPTL3 Monoclonal Antibody (Catalog # MAB38291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Angiopoietin‑like Protein 3/ANGPTL3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3829) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Detection of Angiopoietin-like 3 by Western Blot

ANGPTL4/8-mediated plasmin generation blocks ANGPTL3/8-mediated LPL inhibition. A: LPL stable expression cells were incubated with lipase substrate after being previously incubated with vehicle alone, ANGPTL4/8, tPA + plasminogen, or ANGPTL4/8 + tPA + plasminogen. LPL activity was calculated as a percent of vehicle control. Results are shown as the mean ± SD (n = 10 from 4 independent experiments) (∗P < 0.001 vs. vehicle). B: LPL stable expression cells were incubated with vehicle alone throughout the experiment or in the absence or presence of ANGPTL3/8 after being previously incubated with vehicle alone, ANGPTL4/8 alone, tPA + plasminogen, or ANGPTL4/8 + tPA + plasminogen. Afterward, LPL activity was assessed following the addition of fluorescent lipase substrate and calculated as a percent of vehicle control. Results are shown as the mean ± SD (n = 8 from three independent experiments) (∗P < 0.001 vs. ANGPTL3/8 alone). C: Samples from Figure 1B that contained ANGPTL3/8 were assessed using ANGPTL3 antibody Western blotting. Results are representative of three independent experiments, with two replicates for each experiment (total n = 6 from three independent experiments). ANGPTL, angiopoietin-like protein; LPL, lipoprotein lipase; tPA, tissue plasminogen activator. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37666362), licensed under a CC-BY license. Not internally tested by R&D Systems.