Human APJ Antibody

**This product is in the process of discontinuation and the replacement is MAB8561.
(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Stains human APJ transfectants but not the parental cell line.
  • Source
    Monoclonal Mouse IgG3 Clone # 72133
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with APJ
    Met1-Asp380
    Accession # P35414
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    A172 human glioblastoma cell line
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
APJ in Human Brain. APJ was detected in immersion fixed paraffin-embedded sections of human brain (basal ganglia) using 8 µg/mL Human APJ Monoclonal Antibody (Catalog # MAB856) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
APJ in Human Kidney. APJ was detected in immersion fixed paraffin-embedded sections of human kidney using Human APJ Monoclonal Antibody (Catalog # MAB856) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: APJ

APJ is a G-protein coupled receptor that is related to the angiotensin receptor. It is primarily expressed in coronary artery endothelium and binds the endogenous ligand, apelin. APJ can also function as a co-receptor for HIV.

  • Long Name:
    Apelin Receptor/G-protein Coupled Receptor APJ
  • Entrez Gene IDs:
    187 (Human); 23796 (Mouse); 83518 (Rat)
  • Alternate Names:
    AGTRL1; AGTRL1HG11; angiotensin II receptor-like 1; Angiotensin receptor-like 1; apelin receptor; APJ (apelin) receptor; APJ receptor; APJ; APJFLJ96609; APJR; APLNR; FLJ90771; G protein-coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; HG11 orphan receptor; HG11; MGC45246
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Construction of a gammaretrovirus with a novel tropism and wild-type replication kinetics capable of using human APJ as entry receptor.
    Authors: Bahrami, Shervin, Pagh, Kristina, Ejegod, Ditte, Duch, Mogens, Tolstrup, Martin, Pedersen, Finn Sko
    J Virol, 2012;86(19):10621-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Apelin, an endogenous neuronal peptide, protects hippocampal neurons against excitotoxic injury.
    Authors: O'Donnell LA, Agrawal A, Sabnekar P, Dichter MA, Lynch DR, Kolson DL
    J. Neurochem., 2007;102(6):1905-17.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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