Apolipoprotein A2 (ApoA2) is a major protein component of serum HDL. It is produced by the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver. Polymorphisms of ApoA2 are associated with disorders of cholesterol and fatty acid metabolism. ApoA2 can form disulfide-linked 17 kDa homodimers and heterodimers with other apolipoproteins. Mature human ApoA2 shares 96% aa sequence identity with chimpanzee ApoA2 and 48% - 66% aa sequence identity with bovine, equine, mouse, and rat ApoA2.
Human Apolipoprotein A-II/ApoA2 Antibody
R&D Systems | Catalog # MAB4215
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 395906
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Product Specifications
Immunogen
E. coli-derived recombinant human ApoA2
Gln24-Gln100
Accession # P02652
Gln24-Gln100
Accession # P02652
Specificity
Detects human ApoA2 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) ApoA1 or rhApoE.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Human Apolipoprotein A-II/ApoA2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Apolipoprotein A-II/ApoA2
Sample: Recombinant Human Apolipoprotein A-II/ApoA2
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Apolipoprotein A-II/ApoA2
Alternate Names
Alp-2, APOA2, Apolipoprotein AII, Hdl-1
Gene Symbol
APOA2
UniProt
Additional Apolipoprotein A-II/ApoA2 Products
Product Documents for Human Apolipoprotein A-II/ApoA2 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Apolipoprotein A-II/ApoA2 Antibody
For research use only
Related Research Areas
Citations for Human Apolipoprotein A-II/ApoA2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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