Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Apolipoprotein C-II/ApoC2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ApoC2
Thr23-Glu101
Accession # P02655

Specificity

Detects recombinant and endogenous human ApoC2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Apolipoprotein C‑II/ApoC2 Biotinylated Antibody

Detection of Human Apolipoprotein C-II/ApoC2 antibody by Western Blot.

Detection of Human Apolipoprotein C‑II/ApoC2 by Western Blot.

Western blot shows lysates of human plasma and small intestine tissue. PVDF membrane was probed with 0.25 µg/mL of Human Apolipoprotein C-II/ApoC2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4497) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein C-II/ApoC2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human Apolipoprotein C‑II/ApoC2 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: Human plasma and small intestine tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Apolipoprotein C-II/ApoC2

Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents > 90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% aa identical to mouse and dog ProApoC2, respectively.

Alternate Names

APOC2, Apolipoprotein CII

Entrez Gene IDs

344 (Human); 11813 (Mouse); 292697 (Rat)

Gene Symbol

APOC2

UniProt

P02655

Additional Apolipoprotein C-II/ApoC2 Products

Product Documents for Human Apolipoprotein C‑II/ApoC2 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Apolipoprotein C‑II/ApoC2 Biotinylated Antibody

For research use only

Related Research Areas

Obesity

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