Detection of Human Apolipoprotein C‑II/ApoC2 by Western Blot.
Western blot shows lysates of human plasma and small intestine tissue. PVDF membrane was probed with 0.25 µg/mL of Human Apolipoprotein C‑II/ApoC2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4497) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein C-II/ApoC2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: Apolipoprotein C-II/ApoC2
Apolipoprotein C2 (ApoC2/II) is a 8-9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43-51) and an enzyme interaction site (aa 55-78). ProApoC2 represents > 90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23-28). This does not affect activity. Human ProApoC2 is 59% aa identical to mouse and dog ProApoC2, respectively.
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