Detection of Human Apolipoprotein C-II/ApoC2 by Western Blot.
Western blot shows lysates of human small intestine tissue and plasma. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein C-II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for precursor Apolipoprotein C-II/ApoC2 at approximately 12 kDa and processed ApoC2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human Apolipoprotein C‑II/ApoC2 by Simple WesternTM.
Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein C‑II/ApoC2 at approximately 9 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human Apolipoprotein C‑II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein C-II/ApoC2
Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents >90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% and 62% aa identical to mouse and rat ProApoC2, respectively.
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