Human APRIL/TNFSF13 DuoSet ELISA

Name: Human APRIL/TNFSF13 DuoSet ELISA
Brand: R&D Systems
SKU: DY884B
Rating: 5 (1 reviews)

DY884B 12 Citations 1 Review Datasheet / COA / SDS

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DY884B

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DY008: DuoSet ELISA Ancillary Reagent Kit 2

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Human APRIL / TNFSF13 ELISA Standard Curve

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Product Details

Citations (12)

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human APRIL/TNFSF13 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Sufficient Materials

For fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human APRIL / TNFSF13. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Examples

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Human APRIL/TNFSF13 ELISA Standard Curve

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Human APRIL/TNFSF13 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: APRIL/TNFSF13

A proliferation-inducing ligand (APRIL), also known as TALL-2 and TRDL-1, is a type II transmembrane protein that is a member of the TNF ligand superfamily (TNFSF13). APRIL expression is low in normal tissues, but is elevated in several types of tumors and transformed cell lines. APRIL stimulates proliferation of tumor cell lines and increases tumorigenicity in nude mice.

Long Name:

A Proliferation-inducing Ligand

Entrez Gene IDs:

8741 (Human); 69583 (Mouse)

Alternate Names:

A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2

Citations for Human APRIL/TNFSF13 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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B cell maturation antigen-specific CAR T cells are clinically active in multiple myeloma.
Authors: Cohen A, Garfall A, Stadtmauer E, Melenhorst J, Lacey S, Lancaster E, Vogl D, Weiss B, Dengel K, Nelson A, Plesa G, Chen F, Davis M, Hwang W, Young R, Brogdon J, Isaacs R, Pruteanu-Malinici I, Siegel D, Levine B, June C, Milone M
J Clin Invest, 2019;130(0):.
Species: Human
Sample Types: Serum (confirm CAR-T)
Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants
Authors: YJ Park, SJ Woo, YM Kim, S Hong, YE Lee, KH Park
Invest. Ophthalmol. Vis. Sci., 2019;60(12):3813-3820.
Species: Human
Sample Types: Plasma
Altered circulating levels of B cell growth factors and their modulation upon anti-tuberculosis treatment in pulmonary tuberculosis and tuberculous lymphadenitis
Authors: GR Kathamuthu, K Moideen, VV Banurekha, D Nair, R Sridhar, D Baskaran, S Babu
PLoS ONE, 2018;13(11):e0207404.
Species: Human
Sample Types: Plasma
BAFF-R and TACI expression on CD3+ T cells: Interplay among BAFF, APRIL and T helper cytokines profile in systemic lupus erythematosus
Authors: DC Salazar-Ca, P Ortíz-Laza, M Marín-Rosa, A Cruz, F Muñoz-Vall, R Tapia-Llan, G Orozco-Bar, R Machado-Co, CA Palafox-Sá
Cytokine, 2018;0(0):.
Species: Human
Sample Types: Serum
Serum BAFF in Indian patients with IIM: a retrospective study reveals novel clinico-phenotypic associations in children and adults
Authors: L Gupta, S Chaurasia, P Srivastava, S Dwivedi, A Lawrence, R Misra
Clin. Rheumatol., 2018;0(0):.
Species: Human
Sample Types: Serum
Abnormal differentiation of B cells and megakaryocytes in Roifman syndrome
Authors: J Heremans, JE Garcia-Per, E Turro, SM Schlenner, I Casteels, R Collin, F de Zegher, D Greene, S Humblet-Ba, S Lesage, P Matthys, CJ Penkett, K Put, K Stirrups, C Thys, C Van Geet, E Van Nieuwe, C Wouters, I Meyts, K Freson, A Liston,
J. Allergy Clin. Immunol., 2018;0(0):.
Species: Human
Sample Types: Serum
Modulation of Mycobacterium tuberculosis-specific humoral immune responses is associated with Strongyloides stercoralis co-infection
Authors: R Anuradha, S Munisankar, Y Bhootra, C Dolla, P Kumaran, TB Nutman, S Babu
PLoS Negl Trop Dis, 2017;11(5):e0005569.
Species: Human
Sample Types: Plasma
Urinary B cell Activating Factor (BAFF) and A Proliferation Inducing Ligand (APRIL): Potential biomarkers of Active Lupus Nephritis
Authors: Ramnath Misra
Clin. Exp. Immunol., 2016;0(0):.
Species: Human
Sample Types: Serum
Inflamm-aging influences immune cell survival factors in human bone marrow
Eur. J. Immunol, 2016;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Utility of CSF Cytokine/Chemokines as Markers of Active Intrathecal Inflammation: Comparison of Demyelinating, Anti-NMDAR and Enteroviral Encephalitis
PLoS ONE, 2016;11(8):e0161656.
Species: Human
Sample Types: CSF

Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway.
Authors: Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D
PLoS ONE, 2013;8(12):e82867.
Species: Human
Sample Types: Cell Culture Supernates

Study of the quantitative, functional, cytogenetic, and immunoregulatory properties of bone marrow mesenchymal stem cells in patients with B-cell chronic lymphocytic leukemia.
Authors: Pontikoglou C, Kastrinaki M, Klaus M, Kalpadakis C, Katonis P, Alpantaki K, Pangalis G, Papadaki H
Stem Cells Dev, 2013;22(9):1329-41.
Species: Human
Sample Types: Cell Culture Supernates