Human APRIL/TNFSF13 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Data Examples
Product Datasheets
Preparation and Storage
Background: APRIL/TNFSF13
A proliferation-inducing ligand (APRIL), also known as TALL-2 and TRDL-1, is a type II transmembrane protein that is a member of the TNF ligand superfamily (TNFSF13). APRIL expression is low in normal tissues, but is elevated in several types of tumors and transformed cell lines. APRIL stimulates proliferation of tumor cell lines and increases tumorigenicity in nude mice.
Citations for Human APRIL/TNFSF13 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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B cell maturation antigen-specific CAR T cells are clinically active in multiple myeloma.
Authors: Cohen A, Garfall A, Stadtmauer E, Melenhorst J, Lacey S, Lancaster E, Vogl D, Weiss B, Dengel K, Nelson A, Plesa G, Chen F, Davis M, Hwang W, Young R, Brogdon J, Isaacs R, Pruteanu-Malinici I, Siegel D, Levine B, June C, Milone M
J Clin Invest, 2019;130(0):.
Species: Human
Sample Types: Serum (confirm CAR-T)
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Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants
Authors: YJ Park, SJ Woo, YM Kim, S Hong, YE Lee, KH Park
Invest. Ophthalmol. Vis. Sci., 2019;60(12):3813-3820.
Species: Human
Sample Types: Plasma
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Altered circulating levels of B cell growth factors and their modulation upon anti-tuberculosis treatment in pulmonary tuberculosis and tuberculous lymphadenitis
Authors: GR Kathamuthu, K Moideen, VV Banurekha, D Nair, R Sridhar, D Baskaran, S Babu
PLoS ONE, 2018;13(11):e0207404.
Species: Human
Sample Types: Plasma
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BAFF-R and TACI expression on CD3+ T cells: Interplay among BAFF, APRIL and T helper cytokines profile in systemic lupus erythematosus
Authors: DC Salazar-Ca, P Ortíz-Laza, M Marín-Rosa, A Cruz, F Muñoz-Vall, R Tapia-Llan, G Orozco-Bar, R Machado-Co, CA Palafox-Sá
Cytokine, 2018;0(0):.
Species: Human
Sample Types: Serum
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Serum BAFF in Indian patients with IIM: a retrospective study reveals novel clinico-phenotypic associations in children and adults
Authors: L Gupta, S Chaurasia, P Srivastava, S Dwivedi, A Lawrence, R Misra
Clin. Rheumatol., 2018;0(0):.
Species: Human
Sample Types: Serum
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Abnormal differentiation of B cells and megakaryocytes in Roifman syndrome
Authors: J Heremans, JE Garcia-Per, E Turro, SM Schlenner, I Casteels, R Collin, F de Zegher, D Greene, S Humblet-Ba, S Lesage, P Matthys, CJ Penkett, K Put, K Stirrups, C Thys, C Van Geet, E Van Nieuwe, C Wouters, I Meyts, K Freson, A Liston,
J. Allergy Clin. Immunol., 2018;0(0):.
Species: Human
Sample Types: Serum
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Modulation of Mycobacterium tuberculosis-specific humoral immune responses is associated with Strongyloides stercoralis co-infection
Authors: R Anuradha, S Munisankar, Y Bhootra, C Dolla, P Kumaran, TB Nutman, S Babu
PLoS Negl Trop Dis, 2017;11(5):e0005569.
Species: Human
Sample Types: Plasma
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Urinary B cell Activating Factor (BAFF) and A Proliferation Inducing Ligand (APRIL): Potential biomarkers of Active Lupus Nephritis
Authors: Ramnath Misra
Clin. Exp. Immunol., 2016;0(0):.
Species: Human
Sample Types: Serum
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Inflamm-aging influences immune cell survival factors in human bone marrow
Eur. J. Immunol, 2016;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Utility of CSF Cytokine/Chemokines as Markers of Active Intrathecal Inflammation: Comparison of Demyelinating, Anti-NMDAR and Enteroviral Encephalitis
PLoS ONE, 2016;11(8):e0161656.
Species: Human
Sample Types: CSF
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Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway.
Authors: Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D
PLoS ONE, 2013;8(12):e82867.
Species: Human
Sample Types: Cell Culture Supernates
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Study of the quantitative, functional, cytogenetic, and immunoregulatory properties of bone marrow mesenchymal stem cells in patients with B-cell chronic lymphocytic leukemia.
Authors: Pontikoglou C, Kastrinaki M, Klaus M, Kalpadakis C, Katonis P, Alpantaki K, Pangalis G, Papadaki H
Stem Cells Dev, 2013;22(9):1329-41.
Species: Human
Sample Types: Cell Culture Supernates
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