Human ARA54 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human ARA54 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human ARA54
    Met127-Gln261
    Accession # Q9UBS8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ARA54 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human ARA54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5124) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ARA54 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ARA54

ARA54 (androgen receptor associated protein 54; also RING finger protein 14) is a 50‑55 kDa member of the zinc finger superfamily of proteins. It is ubiquitously expressed, and has two functions; one is to serve as a homodimeric co‑activator for androgen receptor‑induced transcription, and a second as an E3 ubiquitin-protein ligase. Human ARA54 is 474 amino acids (aa) in length and contains an RDW domain that interacts with E2 ubiquitin ligase (aa 11‑137), two zinc finger domains that qualify as RING-type (aa 220‑266) and IBR-type (aa 289‑350), and an androgen-interaction region (aa 361‑474) that contains a coiled‑coil domain. There is one alternate start site at Met127. Over aa 127‑261, human ARA54 is 87% aa identical to mouse ARA54.

  • Long Name:
    Androgen Receptor Associated Protein 54
  • Entrez Gene IDs:
    9604 (Human); 56736 (Mouse)
  • Alternate Names:
    Androgen receptor-associated protein 54; ARA54; ARA54androgen receptor associated protein 54; EC 6.3.2.-; HFB30; HFB30E3 ubiquitin-protein ligase RNF14; ring finger protein 14FLJ26004; RNF14; Triad2 protein; TRIAD2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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