Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ATRIP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ATRIP
Glu461-Gly791
Accession # Q8WXE1

Specificity

Detects endogenous human ATRIP in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ATRIP Antibody

Detection of Human ATRIP antibody by Western Blot.

Detection of Human ATRIP by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line, MCF-7 human breast cancer cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human ATRIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1579) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATRIP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human and Mouse ATRIP antibody by Simple WesternTM.

Detection of Human and Mouse ATRIP by Simple WesternTM.

Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and LL/2 mouse Lewis lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATRIP at approximately 100 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human ATRIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1579) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human ATRIP Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: MCF‑7 human breast cancer cell line and LL/2 mouse Lewis lung carcinoma cell line

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line, MCF-7 human breast cancer cell line and PC-3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ATRIP

ATRIP (ATR interacting protein) is an 85‑90 kDa member of the ATRIP family of proteins. It is ubiquitously expressed, and recruits ATR to sites of DNA damage and replication stress. Human ATRIP is 791 amino acids (aa) in length. It contains a PRA-ssDNA binding region (aa 1‑107), a coiled-coil domain (aa 108‑217) and an ATR‑recruitment region (aa 641‑726). The coiled-coil region mediates ATRIP oligomerization, and multimerization with ATR, creating complexes of 1000 kDa. ATRIP undergoes phosphorylation at Ser224, Ser239 and Ser518 influencing its role in the DNA damage response. There is an alternate start site at Met94, plus a deletion of aa 658‑684 in a second isoform, and a deletion of aa 661‑687 in a third isoform. Over aa 461‑791, human ATRIP is 76% aa identical to mouse ATRIP.

Long Name

Ataxia Telangiectasia and Rad 3-related Interacting Protein

Alternate Names

TREX1

Entrez Gene IDs

84126 (Human); 235610 (Mouse)

Gene Symbol

ATRIP

UniProt

Q8WXE1

Additional ATRIP Products

Product Documents for Human ATRIP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ATRIP Antibody

For research use only

Citations for Human ATRIP Antibody

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