Human ATRIP Antibody

Catalog # Availability Size / Price Qty
AF1579
AF1579-SP
Detection of Human ATRIP by Western Blot.
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Product Details
Citations (2)
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Human ATRIP Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human ATRIP in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ATRIP
Glu461-Gly791
Accession # Q8WXE1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ATRIP antibody by Western Blot. View Larger

Detection of Human ATRIP by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, MCF-7 human breast cancer cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human ATRIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1579) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATRIP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human and Mouse ATRIP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse ATRIP by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and LL/2 mouse Lewis lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATRIP at approximately 100 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human ATRIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1579) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATRIP

ATRIP (ATR interacting protein) is an 85‑90 kDa member of the ATRIP family of proteins. It is ubiquitously expressed, and recruits ATR to sites of DNA damage and replication stress. Human ATRIP is 791 amino acids (aa) in length. It contains a PRA-ssDNA binding region (aa 1‑107), a coiled-coil domain (aa 108‑217) and an ATR‑recruitment region (aa 641‑726). The coiled-coil region mediates ATRIP oligomerization, and multimerization with ATR, creating complexes of 1000 kDa. ATRIP undergoes phosphorylation at Ser224, Ser239 and Ser518 influencing its role in the DNA damage response. There is an alternate start site at Met94, plus a deletion of aa 658‑684 in a second isoform, and a deletion of aa 661‑687 in a third isoform. Over aa 461‑791, human ATRIP is 76% aa identical to mouse ATRIP.

Long Name
Ataxia Telangiectasia and Rad 3-related Interacting Protein
Entrez Gene IDs
84126 (Human); 235610 (Mouse)
Alternate Names
AGS1; ATM and Rad3-related-interacting protein; ATR interacting protein; ATR-interacting protein; ATRIP; DKFZp762J2115; FLJ12343; MGC20625; MGC21482; MGC26740; TREX1

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Citations for Human ATRIP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Nonfucosylated anti-CD20 antibody potentially induces apoptosis in lymphoma cells through enhanced interaction with FcgammaRIIIb on neutrophils.
    Authors: Nakagawa T, Natsume A, Satoh M, Niwa R
    Leuk. Res., 2010-05-01;34(5):666-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. PHF11 promotes DSB resection, ATR signaling, and HR
    Authors: Gong Y, Handa N, Kowalczykowski SC, de Lange T.
    Genes Dev

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