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Human BAFF / BLyS / TNFSF13B ELISA Standard Curve
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Citations (10)
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Human BAFF/BLyS/TNFSF13B DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
39.1 - 2,500 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human BAFF / BLyS / TNFSF13B. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human BAFF / BLyS / TNFSF13B ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BAFF/BLyS/TNFSF13B

BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.

Long Name:
B cell Activating Factor
Entrez Gene IDs:
10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names:
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

Citations for Human BAFF/BLyS/TNFSF13B DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Characterization of B- and T-Cell Compartment and B-Cell Related Factors Belonging to the TNF/TNFR Superfamily in Patients With Clinically Active Systemic Lupus Erythematosus: Baseline BAFF Serum Levels Are the Strongest Predictor of Response to Belimumab after Twelve Months of Therapy
    Authors: S Piantoni, F Regola, S Masneri, M Merletti, T Lowin, P Airò, A Tincani, F Franceschi, L Andreoli, G Pongratz
    Frontiers in Pharmacology, 2021;12(0):666971.
    Species: Human
    Sample Types: Serum
  2. The relationship between BAFF serum levels, anti-NMDAR autoantibodies and fatigue in patients with systemic lupus erythematosus and multiple sclerosis
    Authors: F Basta, T Möckel, J Petersohn, M Meineck, K Triantafyl, S Engel, A Weinmann, F Luessi, J Weinmann-M, A Schwarting
    Autoimmunity reviews, 2021;20(5):102802.
    Species: Human
    Sample Types: Serum
  3. BAFF, involved in B cell activation through the NF-&kappaB pathway, is related to disease activity and bone destruction in rheumatoid arthritis
    Authors: LL Zhang, H Xiao, F Zhang, YJ Wu, JL Shu, Y Li, Y Tai, SQ Xu, JH Xu, W Wei
    Acta pharmacologica Sinica, 2021;0(0):.
    Species: Human
    Sample Types: Serum
  4. Plasma biomarkers of the amyloid pathway are associated with geographic atrophy secondary to age-related macular degeneration
    Authors: K Lashkari, GC Teague, U Beattie, J Betts, S Kumar, MM McLaughlin, FJ López
    PLoS ONE, 2020;15(8):e0236283.
    Species: Human
    Sample Types: Plasma
  5. B cell maturation antigen-specific CAR T cells are clinically active in multiple myeloma.
    Authors: Cohen A, Garfall A, Stadtmauer E, Melenhorst J, Lacey S, Lancaster E, Vogl D, Weiss B, Dengel K, Nelson A, Plesa G, Chen F, Davis M, Hwang W, Young R, Brogdon J, Isaacs R, Pruteanu-Malinici I, Siegel D, Levine B, June C, Milone M
    J Clin Invest, 2019;130(0):.
    Species: Human
    Sample Types: Serum (confirm CAR-T)
  6. Altered circulating levels of B cell growth factors and their modulation upon anti-tuberculosis treatment in pulmonary tuberculosis and tuberculous lymphadenitis
    Authors: GR Kathamuthu, K Moideen, VV Banurekha, D Nair, R Sridhar, D Baskaran, S Babu
    PLoS ONE, 2018;13(11):e0207404.
    Species: Human
    Sample Types: Plasma
  7. IFN type I and II induce BAFF secretion from human decidual stromal cells
    Authors: AC Lundell, I Nordstr”m, K Andersson, C Lundqvist, E Telemo, S Nava, H Kaipe, A Rudin
    Sci Rep, 2017;7(0):39904.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Urinary B cell Activating Factor (BAFF) and A Proliferation Inducing Ligand (APRIL): Potential biomarkers of Active Lupus Nephritis
    Authors: Ramnath Misra
    Clin. Exp. Immunol., 2016;0(0):.
    Species: Human
    Sample Types: Urine
  9. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2016;30(1):163-72.
    Species: Human
    Sample Types: Serum
  10. Utility of CSF Cytokine/Chemokines as Markers of Active Intrathecal Inflammation: Comparison of Demyelinating, Anti-NMDAR and Enteroviral Encephalitis
    PLoS ONE, 2016;11(8):e0161656.
    Species: Human
    Sample Types: CSF


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