|Detection of Human BCR by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human BCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5129) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BCR at approximately 155 - 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
BCR (Breakpoint cluster region protein) is a novel 155‑165 kDa Ser/Thr protein kinase. It is found in fibroblasts and hematopoietic cells, interacts with 14-3-3, and serves as a natural inhibitor of the growth factor-associated c-Abl nonreceptor protein tyrosine kinase. Human BCR is 1271 amino acids (aa) in length. It contains a coiled-coil dimerization motif (aa 28‑68), a Ser/Thr kinase region (aa 176‑426), one DH domain (aa 498‑691) and a Rho-Gap region (aa 1054‑1248). The N-terminal aa 1‑902 and 1‑927 are known to contribute to the BCR‑ABL oncoprotein. BCR likely forms homodimers and 650 kDa homotetramers. An alternate start site exists at Met498, while other isoforms show a deletion of aa 961‑1004, plus a 61 and 37 aa substitution for aa 1‑488. Over aa 1‑426, human BCR is 89% aa identical to mouse BCR.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided