Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all BCR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BCR
Lys174-Asp331
Accession # P11274

Specificity

Detects human BCR in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human BCR Antibody

Detection of Human BCR antibody by Western Blot.

Detection of Human BCR by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human BCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5129) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BCR at approximately 155 - 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human BCR Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BCR

BCR (Breakpoint cluster region protein) is a novel 155‑165 kDa Ser/Thr protein kinase. It is found in fibroblasts and hematopoietic cells, interacts with 14-3-3, and serves as a natural inhibitor of the growth factor-associated c-Abl nonreceptor protein tyrosine kinase. Human BCR is 1271 amino acids (aa) in length. It contains a coiled-coil dimerization motif (aa 28‑68), a Ser/Thr kinase region (aa 176‑426), one DH domain (aa 498‑691) and a Rho-Gap region (aa 1054‑1248). The N-terminal aa 1‑902 and 1‑927 are known to contribute to the BCR‑ABL oncoprotein. BCR likely forms homodimers and 650 kDa homotetramers. An alternate start site exists at Met498, while other isoforms show a deletion of aa 961‑1004, plus a 61 and 37 aa substitution for aa 1‑488. Over aa 1‑426, human BCR is 89% aa identical to mouse BCR.

Long Name

Breakpoint Cluster Region

Alternate Names

ALL, PHL

Entrez Gene IDs

613 (Human); 110279 (Mouse); 309696 (Rat)

Gene Symbol

BCR

UniProt

P11274

Additional BCR Products

Product Documents for Human BCR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human BCR Antibody

For research use only

Related Research Areas

Intracellular Kinases
NFkB Pathway

Citations for Human BCR Antibody

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