Human BCR Antibody

Catalog # Availability Size / Price Qty
Detection of Human BCR by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human BCR Antibody Summary

Species Reactivity
Detects human BCR in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human BCR
Accession # P11274
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BCR antibody by Western Blot. View Larger

Detection of Human BCR by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human BCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5129) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BCR at approximately 155 - 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCR

BCR (Breakpoint cluster region protein) is a novel 155‑165 kDa Ser/Thr protein kinase. It is found in fibroblasts and hematopoietic cells, interacts with 14-3-3, and serves as a natural inhibitor of the growth factor-associated c-Abl nonreceptor protein tyrosine kinase. Human BCR is 1271 amino acids (aa) in length. It contains a coiled-coil dimerization motif (aa 28‑68), a Ser/Thr kinase region (aa 176‑426), one DH domain (aa 498‑691) and a Rho-Gap region (aa 1054‑1248). The N-terminal aa 1‑902 and 1‑927 are known to contribute to the BCR‑ABL oncoprotein. BCR likely forms homodimers and 650 kDa homotetramers. An alternate start site exists at Met498, while other isoforms show a deletion of aa 961‑1004, plus a 61 and 37 aa substitution for aa 1‑488. Over aa 1‑426, human BCR is 89% aa identical to mouse BCR.

Long Name
Breakpoint Cluster Region
Entrez Gene IDs
613 (Human); 110279 (Mouse); 309696 (Rat)
Alternate Names
ALL; ALLD22S11; BCR; BCR1; breakpoint cluster region protein; breakpoint cluster region; CMLEC; D22S662; PHL; PHLFLJ16453; Renal carcinoma antigen NY-REN-26

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Citation for Human BCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia
    Authors: L Löf, L Arngården, U Olsson-Str, B Siart, M Jansson, JS Dahlin, I Thörn, L Christians, M Hermansson, A Larsson, E Ahlstrand, G Wålinder, O Söderberg, R Rosenquist, U Landegren, M Kamali-Mog
    Sci Rep, 2017;7(1):623.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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