Human BCR Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human BCR in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human BCR
    Lys174-Asp331
    Accession # P11274
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human BCR by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human BCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5129) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BCR at approximately 155 - 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BCR

BCR (Breakpoint cluster region protein) is a novel 155‑165 kDa Ser/Thr protein kinase. It is found in fibroblasts and hematopoietic cells, interacts with 14-3-3, and serves as a natural inhibitor of the growth factor-associated c-Abl nonreceptor protein tyrosine kinase. Human BCR is 1271 amino acids (aa) in length. It contains a coiled-coil dimerization motif (aa 28‑68), a Ser/Thr kinase region (aa 176‑426), one DH domain (aa 498‑691) and a Rho-Gap region (aa 1054‑1248). The N-terminal aa 1‑902 and 1‑927 are known to contribute to the BCR‑ABL oncoprotein. BCR likely forms homodimers and 650 kDa homotetramers. An alternate start site exists at Met498, while other isoforms show a deletion of aa 961‑1004, plus a 61 and 37 aa substitution for aa 1‑488. Over aa 1‑426, human BCR is 89% aa identical to mouse BCR.

  • Long Name:
    Breakpoint Cluster Region
  • Entrez Gene IDs:
    613 (Human); 110279 (Mouse); 309696 (Rat)
  • Alternate Names:
    ALL; ALLD22S11; BCR; BCR1; breakpoint cluster region protein; breakpoint cluster region; CMLEC 2.7.11.1; D22S662; PHL; PHLFLJ16453; Renal carcinoma antigen NY-REN-26
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia
    Authors: L Löf, L Arngården, U Olsson-Str, B Siart, M Jansson, JS Dahlin, I Thörn, L Christians, M Hermansson, A Larsson, E Ahlstrand, G Wålinder, O Söderberg, R Rosenquist, U Landegren, M Kamali-Mog
    Sci Rep, 2017;7(1):623.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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