Human beta-Glucuronidase/GUSB Antibody

Catalog # Availability Size / Price Qty
AF6144
AF6144-SP
Detection of Human  beta ‑Glucuronidase/GUSB by Western Blot.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human beta-Glucuronidase/GUSB Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta ‑Glucuronidase/GUSB in direct ELISAs and Western blots.  
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta ‑Glucuronidase/GUSB
Met1-Thr651
Accession # AAA52561
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human  beta -Glucuronidase/GUSB antibody by Western Blot. View Larger

Detection of Human beta ‑Glucuronidase/GUSB by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human beta -Glucuronidase/GUSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6144) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for beta -Glucuronidase/GUSB at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Human  beta -Glucuronidase/GUSB antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human beta ‑Glucuronidase/GUSB by Simple WesternTM. Simple Western lane view shows lysates of HT-29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta -Glucuronidase/GUSB at approximately 92 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human beta -Glucuronidase/GUSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6144) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Glucuronidase/GUSB

Human beta -Glucuronidase (EC 3.2.1.31) encoded by the GUSB gene is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans that include heparan sulfate, chondroitin sulfate and hyaluronan (1). The enzyme is only active on the glucuronic acid of the non-reducing end. The native protein has been reported as a tetrameric glycoprotein composed of identical subunits (1, 2). Mutations in the GUSB gene are linked to mucopolysaccharidosis type VII (3). Accumulation of partially degraded glycosaminoglycans, with glucuronic acid residues at the non-reducing termini, are usually found in the lysosomes of patients with the disease (3). It has also been reported that this enzyme may contribute to the depletion of chondroitin from cartilage and thereby facilitate the damage of joints in rheumatoid arthritis (4).

References
  1. Shipley, J.M. et al. (1993) Am. J. Hum. Genet. 52:517.
  2. Oshima, A, et al. (1987) Proc. Natl. Acad. Sci. USA 84:685.
  3. Bell, C.E. Jr. et al. (1977) J. Clin. Invest. 59:97.
  4. Ortutay, Z. et al. (2003) Arthritis Rheum. 48:2163.
Entrez Gene IDs
2990 (Human)
Alternate Names
beta-D-glucuronidase; Beta-G1; beta-glucuronidase; BG; EC 3.2.1.31; FLJ39445; glucuronidase, beta; GUSB; MPS7

Product Datasheets

You must select a language.

x

Citations for Human beta-Glucuronidase/GUSB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Upregulation of C/EBP Homologous Protein induced by ER Stress Mediates Epithelial to Myofibroblast Transformation in ADTKD-UMOD
    Authors: D Wang, Y Qiu, J Fan, Y Liu, W Chen, Z Li, W Chen, X Wang
    International Journal of Medical Sciences, 2022;19(2):364-376.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer.
    Authors: Ward, S T, Li, K K, Hepburn, E, Weston, C J, Curbishley, S M, Reynolds, G M, Hejmadi, R K, Bicknell, R, Eksteen, B, Ismail, T, Rot, A, Adams, D H
    Br J Cancer, 2015;112(2):319-28.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human beta-Glucuronidase/GUSB Antibody

There are currently no reviews for this product. Be the first to review Human beta-Glucuronidase/GUSB Antibody and earn rewards!

Have you used Human beta-Glucuronidase/GUSB Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review