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Human BID Antibody

Catalog #: AF846
6 Citations Datasheet / COA / SDS
Newer Version Available: MAB860
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Discontinued Product

AF846 has been discontinued and is replaced by AF860. MAB860.

Detection of Human BID by Western Blot.
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Product Details
Citations (6)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BID Antibody Summary

Species Reactivity
Human
Specificity
Detects human BID in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse BID is observed.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
KLH-coupled human BID synthetic peptide
RRELDALGHELPVLAPQWEC
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BID antibody by Western Blot. View Larger

Detection of Human BID by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µg/mL anti-Fas Antibody for 6 hours and WS-1 human fetal skin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human BID Polyclonal Antibody (Catalog # AF846), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BID at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BID

Bid is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). Bid is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells Bid is cytosolic. In response to Fas ligand or TNF, Bid is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of Bid by caspase-8 generates a new N-terminous that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target Bid to the mitochondria (4). Bid may then interact withanother pro-apoptotic Bcl-2 family member Bak (5). Interaction of Bid with Bak causes altered mitochondrial membrane permeability. A (9-13) amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the Bid interaction with other Bcl-2 family members. Bid is neutralized by binding to the anti‑apoptotic member Bcl-xL.

 

References
  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Luo, X., et al. (1998) Cell 94:481.
  3. Li, H. et al. (1998) Cell 94:491.
  4. Zha, J. et al. (2000) Science 290:1761.
  5. Wei, M.C. et al. (2000) Genes Dev. 14:2060.
Long Name
BH3 Interacting Domain Death Agonist
Entrez Gene IDs
637 (Human); 12122 (Mouse)
Alternate Names
apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID; tbid

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Citations for Human BID Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells
    Authors: A Willms, H Schittek, S Rahn, J Sosna, U Mert, D Adam, A Trauzold
    PLoS ONE, 2019-04-04;14(4):e0214847.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment
    Authors: A Mohr, L Deedigan, S Jencz, Y Mehrabadi, L Houlden, SM Albarenque, RM Zwacka
    Cell Death Differ., 2017-11-03;0(0):.
  3. Caspase-2-mediated cell death is required for deleting aneuploid cells
    Oncogene, 2016-12-19;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8.
    Authors: Ikner A, Ashkenazi A
    J. Biol. Chem., 2011-04-27;286(24):21546-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Enhancement of stress-induced apoptosis in B-lineage cells by caspase-9 inhibitor.
    Authors: Shah N, Asch RJ, Lysholm AS, Lebien TW
    Blood, 2004-07-08;104(9):2873-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Endoplasmic reticulum calcium pool depletion-induced apoptosis is coupled with activation of the death receptor 5 pathway.
    Authors: He Q, Lee DI, Rong R, Yu M, Luo X, Klein M, El-Deiry WS, Huang Y, Hussain A, Sheikh MS
    Oncogene, 2002-04-18;21(17):2623-33.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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