Human/Mouse BID Antibody

Catalog # Availability Size / Price Qty
AF860
AF860-SP
Human/Mouse BID Antibody in Data
3 Images
Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse BID Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse BID, a fragment generated in cells treated with anti-Fas and the carboxyl‑terminal portion of recombinant BID generated by cleavage with Caspase 8 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse BID
Met1-Asp195
Accession # AAC71064
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunoprecipitation
4 µg/106 cells
Jurkat human acute T cell leukemia cell line treated with anti-Fas, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human BID by Western Blot. View Larger

Detection of Human BID by Western Blot. Western blot shows Jurkat human acute T cell leukemia cell line treated with apoptosis inducer anti-Fas for the indicated times. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BID at approximately 20 kDa (as indicated). For additional reference short (5 seconds, left panel) and long (60 seconds, right panel) exposures are shown. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot Detection of Human and Mouse BID by Western Blot. View Larger

Detection of Human and Mouse BID by Western Blot. Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human BID by Simple Western<sup>TM</sup>. View Larger

Detection of Human BID by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for BID at approximately 29 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BID

BID is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). BID is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells BID is cytosolic. In response to Fas ligand or TNF, BID is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of BID by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target BID to the mitochondria (4). BID may then interact with another pro-apoptotic Bcl-2 family member Bak (5). Interaction of BID with Bak causes altered mitochondrial membrane permeability. A 9‑13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the BID interaction with other Bcl-2 family members. BID is neutralized by binding to the anti-apoptotic member Bcl-xL.

References
  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Luo, X., et al. (1998) Cell 94:481.
  3. Li, H. et al. (1998) Cell 94:491.
  4. Zha, J. et al. (2000) Science 290:1761.
  5. Wei, M.C. et al. (2000) Genes Dev. 14:2060.
Long Name
BH3 Interacting Domain Death Agonist
Entrez Gene IDs
637 (Human); 12122 (Mouse)
Alternate Names
apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID; tbid

Product Datasheets

Citations for Human/Mouse BID Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture
    Authors: NM de Vasconc, N Van Opdenb, H Van Gorp, E Parthoens, M Lamkanfi
    Cell Death Differ., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism
    Authors: S Naghdi, WS Slovinsky, M Madesh, E Rubin, G Hajnóczky
    Cell Death Dis, 2018;9(10):1028.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Executioner caspases and CAD are essential for mutagenesis induced by TRAIL or vincristine
    Authors: MA Miles, CJ Hawkins
    Cell Death Dis, 2017;8(10):e3062.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Highly tumorigenic hepatocellular carcinoma cell line with cancer stem cell-like properties
    Authors: B Lacoste, VA Raymond, S Cassim, P Lapierre, M Bilodeau
    PLoS ONE, 2017;12(2):e0171215.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Germline quality control: eEF2K stands guard to eliminate defective oocytes.
    Authors: Chu H, Liao Y, Novak J, Hu Z, Merkin J, Shymkiv Y, Braeckman B, Dorovkov M, Nguyen A, Clifford P, Nagele R, Harrison D, Ellis R, Ryazanov A
    Dev Cell, 2014;28(5):561-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.
    Authors: Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, Kaufmann S, Gores G
    J Biol Chem, 2014;289(33):22835-49.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. GLI3-dependent repression of DR4 mediates hedgehog antagonism of TRAIL-induced apoptosis.
    Authors: Kurita S, Mott JL, Almada LL
    Oncogene, 2010;29(34):4848-58.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Down-regulation of FoxO-dependent c-FLIP expression mediates TRAIL-induced apoptosis in activated hepatic stellate cells.
    Authors: Park SJ, Sohn HY, Yoon J, Park SI
    Cell. Signal., 2009;21(10):1495-503.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP.
    Authors: Park SJ, Kim MJ, Kim HB, Kang CD, Kim SH
    Biochem. J., 2009;420(1):73-81.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Cardioprotection against myocardial infarction with PTD-BIR3/RING, a XIAP mimicking protein.
    Authors: Souktani R, Pons S, Guegan C, Bouhidel O, Bruneval P, Zini R, Mandet C, Onteniente B, Berdeaux A, Ghaleh B
    J. Mol. Cell. Cardiol., 2009;46(5):713-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Trichostatin A sensitizes human ovarian cancer cells to TRAIL-induced apoptosis by down-regulation of c-FLIPL via inhibition of EGFR pathway.
    Authors: Park SJ, Kim MJ, Kim HB, Sohn HY, Bae JH, Kang CD, Kim SH
    Biochem. Pharmacol., 2009;77(8):1328-36.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Yersinia YopP-induced apoptotic cell death in murine dendritic cells is partially independent from action of caspases and exhibits necrosis-like features.
    Authors: Grobner S, Autenrieth SE, Soldanova I, Gunst DS, Schaller M, Bohn E, Muller S, Leverkus M, Wesselborg S, Autenrieth IB, Borgmann S
    Apoptosis, 2006;11(11):1959-68.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  13. Translocation of full-length Bid to mitochondria during anoikis.
    Authors: Valentijn AJ, Gilmore AP
    J. Biol. Chem., 2004;279(31):32848-57.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Early events in the anoikis program occur in the absence of caspase activation.
    Authors: Wang P, Valentijn AJ, Gilmore AP, Streuli CH
    J. Biol. Chem., 2003;278(22):19917-25.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Post-translational modification of Bid has differential effects on its susceptibility to cleavage by caspase 8 or caspase 3.
    Authors: Degli Esposti M, Ferry G, Masdehors P, Boutin JA, Hickman JA, Dive C
    J. Biol. Chem., 2003;278(18):15749-57.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
    Authors: Jayanthi S, Deng X, Bordelon M, McCoy MT, Cadet JL
    FASEB J., 2001;15(10):1745-52.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Human/Mouse BID Antibody

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Human/Mouse BID Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse BID Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human

Human/Mouse BID Antibody
By Anonymous on 11/09/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human