Human/Mouse BID Antibody

Catalog # Availability Size / Price Qty
AF860
AF860-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Human BID by Western Blot.
5 Images
Product Details
Citations (35)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse BID Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse BID, a fragment generated in cells treated with anti-Fas and the carboxyl‑terminal portion of recombinant BID generated by cleavage with Caspase 8 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse BID
Met1-Asp195
Accession # AAC71064
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunoprecipitation
4 µg/106 cells
Jurkat human acute T cell leukemia cell line treated with anti-Fas, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BID antibody by Western Blot. View Larger

Detection of Human BID by Western Blot. Western blot shows Jurkat human acute T cell leukemia cell line treated with apoptosis inducer anti-Fas for the indicated times. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BID at approximately 20 kDa (as indicated). For additional reference short (5 seconds, left panel) and long (60 seconds, right panel) exposures are shown. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western Blot Detection of Human and Mouse BID antibody by Western Blot. View Larger

Detection of Human and Mouse BID by Western Blot. Western blot shows lysates of SVEC4-10 mouse vascular endothelial cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BID at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human BID antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human BID by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for BID at approximately 29 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Human BID by Western Blot View Larger

Detection of Human BID by Western Blot Survivin is predominantly expressed in obese hASCs and is influenced by the inflammatory environment. (a) Survivin protein expression and quantification in total adipose tissue (AT), adipocyte fraction (AD) and stromal-vascular fraction (SVF) from SAT of lean and obese patients. Fumarylacetoacetase (FAA) was used as a loading control. Ponceau S staining is also provided. *P<0.001 versus total AT and AD. (b) Survivin and p53 protein levels and quantification in hASCs from lean and obese patients. GAPDH was used as a loading control. *P<0.01 versus lean. (c) Survivin secreted levels and quantification in medium from lean- and obese-derived hASCs. Ponceau S staining was used to check loading. *P<0.05 versus lean conditioned medium. (d) Total cell extracts of lean- and obese-derived hASCs were subjected to immunoblotting with antibodies against apoptotic and antiapoptotic markers and GAPDH was used as a loading control. No significant differences were found. (e) Lean hASCs were cultured in RPMI medium (C: control) or for 24 h in conditioned medium (CM) from macrophages (M0, M1 and M2) and cells were subjected to immunobloting with survivin and GAPDH antibodies. *P<0.05 versus CM from control, and M0; #P<0.05 versus M2. (f) Left panel, lean hASCs were cultured in RPMI medium (C: control) or RPMI supplemented with IL-1 beta (IL1B) or IL-1 beta and IL-1 beta receptor antagonist (a); right panel, with CM of M1 macrophages or CM of M1 and IL-1 beta receptor antagonist (a). Survivin protein levels were measured and GAPDH was used as a loading control. Quantification of three experiments is shown. *P<0.05 versus control (c); #P<0.05 as indicated in figure. Data information: Values are expressed as mean±S.E.M. Statistical analysis: unpaired Student’s t-test. n=3–4 patients for each group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28518147), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human BID by Western Blot View Larger

Detection of Human BID by Western Blot Survivin is predominantly expressed in obese hASCs and is influenced by the inflammatory environment. (a) Survivin protein expression and quantification in total adipose tissue (AT), adipocyte fraction (AD) and stromal-vascular fraction (SVF) from SAT of lean and obese patients. Fumarylacetoacetase (FAA) was used as a loading control. Ponceau S staining is also provided. *P<0.001 versus total AT and AD. (b) Survivin and p53 protein levels and quantification in hASCs from lean and obese patients. GAPDH was used as a loading control. *P<0.01 versus lean. (c) Survivin secreted levels and quantification in medium from lean- and obese-derived hASCs. Ponceau S staining was used to check loading. *P<0.05 versus lean conditioned medium. (d) Total cell extracts of lean- and obese-derived hASCs were subjected to immunoblotting with antibodies against apoptotic and antiapoptotic markers and GAPDH was used as a loading control. No significant differences were found. (e) Lean hASCs were cultured in RPMI medium (C: control) or for 24 h in conditioned medium (CM) from macrophages (M0, M1 and M2) and cells were subjected to immunobloting with survivin and GAPDH antibodies. *P<0.05 versus CM from control, and M0; #P<0.05 versus M2. (f) Left panel, lean hASCs were cultured in RPMI medium (C: control) or RPMI supplemented with IL-1 beta (IL1B) or IL-1 beta and IL-1 beta receptor antagonist (a); right panel, with CM of M1 macrophages or CM of M1 and IL-1 beta receptor antagonist (a). Survivin protein levels were measured and GAPDH was used as a loading control. Quantification of three experiments is shown. *P<0.05 versus control (c); #P<0.05 as indicated in figure. Data information: Values are expressed as mean±S.E.M. Statistical analysis: unpaired Student’s t-test. n=3–4 patients for each group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28518147), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BID

BID is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). BID is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells BID is cytosolic. In response to Fas ligand or TNF, BID is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of BID by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target BID to the mitochondria (4). BID may then interact with another pro-apoptotic Bcl-2 family member Bak (5). Interaction of BID with Bak causes altered mitochondrial membrane permeability. A 9‑13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the BID interaction with other Bcl-2 family members. BID is neutralized by binding to the anti-apoptotic member Bcl-xL.

References
  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Luo, X., et al. (1998) Cell 94:481.
  3. Li, H. et al. (1998) Cell 94:491.
  4. Zha, J. et al. (2000) Science 290:1761.
  5. Wei, M.C. et al. (2000) Genes Dev. 14:2060.
Long Name
BH3 Interacting Domain Death Agonist
Entrez Gene IDs
637 (Human); 12122 (Mouse)
Alternate Names
apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID; tbid

Product Datasheets

You must select a language.

x

Citations for Human/Mouse BID Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Caspase-2 is essential for proliferation and self-renewal of nucleophosmin-mutated acute myeloid leukemia
    Authors: Sakthivel, D;Brown-Suedel, AN;Keane, F;Huang, S;Sherry, KM;Charendoff, CI;Dunne, KP;Robichaux, DJ;Le, B;Shin, CS;Carisey, AF;Flanagan, JM;Bouchier-Hayes, L;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. High Levels of Progesterone Receptor B in MCF-7 Cells Enable Radical Anti-Tumoral and Anti-Estrogenic Effect of Progestin
    Authors: N Bajalovic, YZ Or, ARE Woo, SH Lee, VCL Lin
    Biomedicines, 2022-08-02;10(8):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. p53-independent induction of p21 fails to control regeneration and hepatocarcinogenesis in a murine liver injury model
    Authors: LE Buitrago-M, S Marhenke, D Becker, R Geffers, T Itzel, A Teufel, H Jaeschke, A Lechel, K Unger, J Markovic, AD Sharma, JU Marquardt, M Saborowski, A Saborowski, A Vogel
    Cellular and Molecular Gastroenterology and Hepatology, 2021-01-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Plants expressing murine pro-apoptotic protein Bid do not have enhanced PCD
    Authors: A Manara, Z Imanifard, L Fracasso, D Bellin, M Crimi
    Bmc Research Notes, 2020-09-21;13(1):450.
    Species: Arabidopsis thaliana
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. An Apoptotic Caspase Network Safeguards Cell Death Induction in Pyroptotic Macrophages
    Authors: NM de Vasconc, N Van Opdenb, H Van Gorp, R Martín-Pér, A Zecchin, P Vandenabee, M Lamkanfi
    Cell Rep, 2020-07-28;32(4):107959.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. Phenotypic selection with an intrabody library reveals an anti-apoptotic function of PKM2 requiring Mitofusin-1
    Authors: T Liu, T Kuwana, H Zhang, MG Vander Hei, RA Lerner, DD Newmeyer
    PLoS Biol., 2019-06-10;17(6):e2004413.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Mitochondrial fusion and Bid-mediated mitochondrial apoptosis are perturbed by alcohol with distinct dependence on its metabolism
    Authors: S Naghdi, WS Slovinsky, M Madesh, E Rubin, G Hajnóczky
    Cell Death Dis, 2018-10-09;9(10):1028.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Bid maintains mitochondrial cristae structure and function and protects against cardiac disease in an integrative genomics study
    Authors: Christi T Salisbury-Ruf, Clinton C Bertram, Aurelia Vergeade, Daniel S Lark, Qiong Shi, Marlene L Heberling et al.
    eLife
  9. Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture
    Authors: NM de Vasconc, N Van Opdenb, H Van Gorp, E Parthoens, M Lamkanfi
    Cell Death Differ., 2018-04-17;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3
    Authors: Moritz M. Gaidt, Thomas S. Ebert, Dhruv Chauhan, Katharina Ramshorn, Francesca Pinci, Sarah Zuber et al.
    Cell
  11. Executioner caspases and CAD are essential for mutagenesis induced by TRAIL or vincristine
    Authors: MA Miles, CJ Hawkins
    Cell Death Dis, 2017-10-05;8(10):e3062.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Highly tumorigenic hepatocellular carcinoma cell line with cancer stem cell-like properties
    Authors: B Lacoste, VA Raymond, S Cassim, P Lapierre, M Bilodeau
    PLoS ONE, 2017-02-02;12(2):e0171215.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Subcellular localization of PUMA regulates its pro-apoptotic activity in Burkitt's lymphoma B cells
    Authors: Gorbatchev Ambroise, Alain Portier, Nathalie Roders, Damien Arnoult, Aimé Vazquez
    Oncotarget
  14. Importance of extended protease substrate recognition motifs in steering BNIP-2 cleavage by human and mouse granzymes B
    Authors: Petra Van Damme, Kim Plasman, Giel Vandemoortele, Veronique Jonckheere, Sebastian Maurer-Stroh, Kris Gevaert
    BMC Biochemistry
  15. Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.
    Authors: Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, Kaufmann S, Gores G
    J Biol Chem, 2014-06-27;289(33):22835-49.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Germline quality control: eEF2K stands guard to eliminate defective oocytes.
    Authors: Chu H, Liao Y, Novak J, Hu Z, Merkin J, Shymkiv Y, Braeckman B, Dorovkov M, Nguyen A, Clifford P, Nagele R, Harrison D, Ellis R, Ryazanov A
    Dev Cell, 2014-02-27;28(5):561-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Systems analysis of apoptosis protein expression allows the case-specific prediction of cell death responsiveness of melanoma cells
    Authors: E Passante, M L Würstle, C T Hellwig, M Leverkus, M Rehm
    Cell Death & Differentiation
  18. Proapoptotic and survival signaling in the neuroretina at early stages of diabetic retinopathy
    Authors: Angela M. Valverde, Soledad Miranda, Marta García-Ramírez, Águeda González-Rodriguez, Cristina Hernández, Rafael Simó
    Mol. Vis
  19. Prognostic impact of the expression/phosphorylation of the BH3-only proteins of the BCL-2 family in glioblastoma multiforme
    Authors: P F Cartron, D Loussouarn, M Campone, S A Martin, F M Vallette
    Cell Death & Disease
  20. Essential role of protein tyrosine phosphatase 1B in obesity-induced inflammation and peripheral insulin resistance during aging
    Authors: Águeda González-Rodríguez, Jose A. Más-Gutierrez, Mercedes Mirasierra, Antonio Fernandez-Pérez, Yong Jin Lee, Hwi Jin Ko et al.
    Aging Cell
  21. BID regulates AIF-mediated caspase-independent necroptosis by promoting BAX activation
    Authors: L Cabon, P Galán-Malo, A Bouharrour, L Delavallée, M-N Brunelle-Navas, H K Lorenzo et al.
    Cell Death & Differentiation
  22. Bid and Bax Are Involved in Granulosa Cell Apoptosis During Follicular Atresia in Porcine Ovaries
    Authors: Takafumi SAI, Yasufumi GOTO, Ryousuke YOSHIOKA, Akihisa MAEDA, Fuko MATSUDA, Miki SUGIMOTO et al.
    Journal of Reproduction and Development
  23. Evaluation of the effect of alpha -defensin human neutrophil peptides on neutrophil apoptosis
    Authors: Isao Nagaoka, Kaori Suzuki, Taisuke Murakami, François Niyonsaba, Hiroshi Tamura, Michimasa Hirata
    International Journal of Molecular Medicine
  24. Glucose deprivation induces an atypical form of apoptosis mediated by caspase-8 in Bax-, Bak-deficient cells
    Authors: A Caro-Maldonado, S W G Tait, S Ramírez-Peinado, J-E Ricci, I Fabregat, D R Green et al.
    Cell Death & Differentiation
  25. GLI3-dependent repression of DR4 mediates hedgehog antagonism of TRAIL-induced apoptosis.
    Authors: Kurita S, Mott JL, Almada LL
    Oncogene, 2010-06-21;29(34):4848-58.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Vinblastine Induces Acute, Cell Cycle Phase–Independent Apoptosis in Some Leukemias and Lymphomas and Can Induce Acute Apoptosis in Others when Mcl-1 Is Suppressed
    Authors: Bethany L. Salerni, Darcy J. Bates, Tina C. Albershardt, Christopher H. Lowrey, Alan Eastman
    Molecular Cancer Therapeutics
  27. Down-regulation of FoxO-dependent c-FLIP expression mediates TRAIL-induced apoptosis in activated hepatic stellate cells.
    Authors: Park SJ, Sohn HY, Yoon J, Park SI
    Cell. Signal., 2009-05-24;21(10):1495-503.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP.
    Authors: Park SJ, Kim MJ, Kim HB, Kang CD, Kim SH
    Biochem. J., 2009-04-28;420(1):73-81.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Cardioprotection against myocardial infarction with PTD-BIR3/RING, a XIAP mimicking protein.
    Authors: Souktani R, Pons S, Guegan C, Bouhidel O, Bruneval P, Zini R, Mandet C, Onteniente B, Berdeaux A, Ghaleh B
    J. Mol. Cell. Cardiol., 2009-02-20;46(5):713-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  30. Trichostatin A sensitizes human ovarian cancer cells to TRAIL-induced apoptosis by down-regulation of c-FLIPL via inhibition of EGFR pathway.
    Authors: Park SJ, Kim MJ, Kim HB, Sohn HY, Bae JH, Kang CD, Kim SH
    Biochem. Pharmacol., 2009-01-24;77(8):1328-36.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Yersinia YopP-induced apoptotic cell death in murine dendritic cells is partially independent from action of caspases and exhibits necrosis-like features.
    Authors: Grobner S, Autenrieth SE, Soldanova I, Gunst DS, Schaller M, Bohn E, Muller S, Leverkus M, Wesselborg S, Autenrieth IB, Borgmann S
    Apoptosis, 2006-11-01;11(11):1959-68.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  32. Translocation of full-length Bid to mitochondria during anoikis.
    Authors: Valentijn AJ, Gilmore AP
    J. Biol. Chem., 2004-05-17;279(31):32848-57.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  33. Early events in the anoikis program occur in the absence of caspase activation.
    Authors: Wang P, Valentijn AJ, Gilmore AP, Streuli CH
    J. Biol. Chem., 2003-03-05;278(22):19917-25.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Post-translational modification of Bid has differential effects on its susceptibility to cleavage by caspase 8 or caspase 3.
    Authors: Degli Esposti M, Ferry G, Masdehors P, Boutin JA, Hickman JA, Dive C
    J. Biol. Chem., 2003-02-21;278(18):15749-57.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
    Authors: Jayanthi S, åÏctÐs, Deng X, Bordelon M, McCoy MT, Cadet JL
    2001-08-01;15(10):1745-52.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse BID Antibody

Average Rating: 4 (Based on 3 Reviews)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse BID Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse BID Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse BID Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human

Human/Mouse BID Antibody
By Carly Garrison on 11/09/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human