Human/Mouse BID Antibody

(13 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse BID, a fragment generated in cells treated with anti-Fas and the carboxyl‑terminal portion of recombinant BID generated by cleavage with Caspase 8 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse BID
    Met1-Asp195
    Accession # AAC71064
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human BID by Western Blot. Western blot shows Jurkat human acute T cell leukemia cell line treated with apoptosis inducer anti-Fas for the indicated times. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BID at approximately 20 kDa (as indicated). For additional reference short (5 seconds, left panel) and long (60 seconds, right panel) exposures are shown. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human BID by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for BID at approximately 29 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BID

BID is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). BID is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells BID is cytosolic. In response to Fas ligand or TNF, BID is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of BID by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target BID to the mitochondria (4). BID may then interact with another pro-apoptotic Bcl-2 family member Bak (5). Interaction of BID with Bak causes altered mitochondrial membrane permeability. A 9‑13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the BID interaction with other Bcl-2 family members. BID is neutralized by binding to the anti-apoptotic member Bcl-xL.

  • References:
    1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
    2. Luo, X., et al. (1998) Cell 94:481.
    3. Li, H. et al. (1998) Cell 94:491.
    4. Zha, J. et al. (2000) Science 290:1761.
    5. Wei, M.C. et al. (2000) Genes Dev. 14:2060.
  • Long Name:
    BH3 Interacting Domain Death Agonist
  • Entrez Gene IDs:
    637 (Human); 12122 (Mouse)
  • Alternate Names:
    apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Highly tumorigenic hepatocellular carcinoma cell line with cancer stem cell-like properties
    Authors: B Lacoste, VA Raymond, S Cassim, P Lapierre, M Bilodeau
    PLoS ONE, 2017;12(2):e0171215.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.
    Authors: Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, Kaufmann S, Gores G
    J Biol Chem, 2014;289(33):22835-49.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Germline quality control: eEF2K stands guard to eliminate defective oocytes.
    Authors: Chu H, Liao Y, Novak J, Hu Z, Merkin J, Shymkiv Y, Braeckman B, Dorovkov M, Nguyen A, Clifford P, Nagele R, Harrison D, Ellis R, Ryazanov A
    Dev Cell, 2014;28(5):561-72.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. GLI3-dependent repression of DR4 mediates hedgehog antagonism of TRAIL-induced apoptosis.
    Authors: Kurita S, Mott JL, Almada LL
    Oncogene, 2010;29(34):4848-58.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP.
    Authors: Park SJ, Kim MJ, Kim HB, Kang CD, Kim SH
    Biochem. J., 2009;420(1):73-81.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Down-regulation of FoxO-dependent c-FLIP expression mediates TRAIL-induced apoptosis in activated hepatic stellate cells.
    Authors: Park SJ, Sohn HY, Yoon J, Park SI
    Cell. Signal., 2009;21(10):1495-503.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Cardioprotection against myocardial infarction with PTD-BIR3/RING, a XIAP mimicking protein.
    Authors: Souktani R, Pons S, Guegan C, Bouhidel O, Bruneval P, Zini R, Mandet C, Onteniente B, Berdeaux A, Ghaleh B
    J. Mol. Cell. Cardiol., 2009;46(5):713-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  8. Trichostatin A sensitizes human ovarian cancer cells to TRAIL-induced apoptosis by down-regulation of c-FLIPL via inhibition of EGFR pathway.
    Authors: Park SJ, Kim MJ, Kim HB, Sohn HY, Bae JH, Kang CD, Kim SH
    Biochem. Pharmacol., 2009;77(8):1328-36.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Yersinia YopP-induced apoptotic cell death in murine dendritic cells is partially independent from action of caspases and exhibits necrosis-like features.
    Authors: Grobner S, Autenrieth SE, Soldanova I, Gunst DS, Schaller M, Bohn E, Muller S, Leverkus M, Wesselborg S, Autenrieth IB, Borgmann S
    Apoptosis, 2006;11(11):1959-68.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  10. Translocation of full-length Bid to mitochondria during anoikis.
    Authors: Valentijn AJ, Gilmore AP
    J. Biol. Chem., 2004;279(31):32848-57.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. Post-translational modification of Bid has differential effects on its susceptibility to cleavage by caspase 8 or caspase 3.
    Authors: Degli Esposti M, Ferry G, Masdehors P, Boutin JA, Hickman JA, Dive C
    J. Biol. Chem., 2003;278(18):15749-57.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  12. Early events in the anoikis program occur in the absence of caspase activation.
    Authors: Wang P, Valentijn AJ, Gilmore AP, Streuli CH
    J. Biol. Chem., 2003;278(22):19917-25.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  13. Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
    Authors: Jayanthi S, Deng X, Bordelon M, McCoy MT, Cadet JL
    FASEB J., 2001;15(10):1745-52.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Expand to show all 13 Citations
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