Human BLIMP1/PRDM1 Alexa Fluor® 488-conjugated Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human BLIMP1/PRDM1 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 646702
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human BLIMP1/PRDM1
    Lys667-Cys789
    Accession # O75626
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC36081G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of BLIMP1/PRDM1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD19+ CD3- cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC36081G) and Mouse Anti-Human CD38 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BLIMP1/PRDM1

Human BLIMP1 (B lymphocyte-induced maturation protein 1, also known as PRDM1) is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 is 89% aa identical to mouse BLIMP1.

  • Long Name:
    B Lymphocyte Induced Maturation Protein
  • Entrez Gene IDs:
    639 (Human); 12142 (Mouse); 309871 (Rat)
  • Alternate Names:
    Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP1; BLIMP-1; BLIMP1MGC118925; blmp-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI-BF1MGC118924; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1; PR-domain zinc finger protein 1; ZNFPR1A1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis
    Authors: DA Rao, MF Gurish, JL Marshall, K Slowikowsk, CY Fonseka, Y Liu, LT Donlin, LA Henderson, K Wei, F Mizoguchi, NC Teslovich, ME Weinblatt, EM Massarotti, JS Coblyn, SM Helfgott, YC Lee, DJ Todd, VP Bykerk, SM Goodman, AB Pernis, LB Ivashkiv, EW Karlson, PA Nigrovic, A Filer, CD Buckley, JA Lederer, S Raychaudhu, MB Brenner
    Nature, 2017;542(7639):110-114.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients.
    Authors: Banko Z, Pozsgay J, Szili D, Toth M, Gati T, Nagy G, Rojkovich B, Sarmay G
    J Immunol, 2017;198(4):1512-1520.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. MAIT cells are licensed through granzyme exchange to kill bacterially sensitized targets.
    Authors: Kurioka, A, Ussher, J E, Cosgrove, C, Clough, C, Fergusson, J R, Smith, K, Kang, Y-H, Walker, L J, Hansen, T H, Willberg, C B, Klenerman, P
    Mucosal Immunol, 2015;8(2):429-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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