Human BLIMP1/PRDM1 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC36081R-025
Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry.
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Human BLIMP1/PRDM1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human BLIMP1/PRDM1 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 646702
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human BLIMP1/PRDM1
Lys667-Cys789
Accession # O75626
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. View Larger

Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Mouse Anti-Human CD38 PE‑conjugated Monoclonal Antibody (Catalog # FAB2404P) and either (A) Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC36081R) or (B) Mouse IgG1 Alexa Fluor 647 Isotype Control (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of BLIMP1/PRDM1 in Human PBMCs gated on CD19+ CD3- cells by Flow Cytometry. View Larger

Detection of BLIMP1/PRDM1 in Human PBMCs gated on CD19+ CD3- cells by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD19+ CD3- cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC36081R) and Mouse Anti-Human CD38 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BLIMP1/PRDM1

Human BLIMP1 (B Lymphocyte-induced Maturation Protein 1), also known as PRDM1, is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 shares 89% aa sequence identity with mouse BLIMP1.

Long Name
B Lymphocyte Induced Maturation Protein
Entrez Gene IDs
639 (Human); 12142 (Mouse); 309871 (Rat)
Alternate Names
Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP1; BLIMP-1; BLIMP1MGC118925; blmp-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI-BF1MGC118924; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1; PR-domain zinc finger protein 1; ZNFPR1A1

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