Human BLIMP1/PRDM1 Alexa Fluor® 647-conjugated Antibody Summary
Accession # O75626
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Mouse Anti-Human CD38 PE‑conjugated Monoclonal Antibody (Catalog # FAB2404P) and either (A) Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC36081R) or (B) Mouse IgG1 Alexa Fluor 647 Isotype Control (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of BLIMP1/PRDM1 in Human PBMCs gated on CD19+ CD3- cells by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD19+ CD3- cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC36081R) and Mouse Anti-Human CD38 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Human BLIMP1 (B Lymphocyte-induced Maturation Protein 1), also known as PRDM1, is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 shares 89% aa sequence identity with mouse BLIMP1.
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