Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Simple Western

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry, Mass Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 646702
View all BLIMP1/PRDM1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BLIMP1/PRDM1
Lys667-Cys789
Accession # O75626

Specificity

Detects human BLIMP1/PRDM1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human BLIMP1/PRDM1 Antibody

Detection of Human BLIMP1/PRDM1 antibody by Western Blot.

Detection of Human BLIMP1/PRDM1 by Western Blot.

Western blot shows lysates of U266 human myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for BLIMP1/PRDM1 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human BLIMP1/PRDM1 antibody by Simple WesternTM.

Detection of Human BLIMP1/PRDM1 by Simple WesternTM.

Simple Western lane view shows lysates of U266 human myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for BLIMP1/PRDM1 at approximately 114 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry.

U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Detection of BLIMP1/PRDM1 in MM.1S cells by Flow Cytometry

MM.1S cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human BLIMP1/PRDM1 Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: U266 human myeloma cell line or MM.1S human myeloma cell line fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012)

Simple Western

10 µg/mL
Sample: U266 human myeloma cell line

Western Blot

1 µg/mL
Sample: U266 human myeloma cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BLIMP1/PRDM1

Human BLIMP1 (B lymphocyte-induced maturation protein 1, also known as PRDM1) is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 is 89% aa identical to mouse BLIMP1.

Long Name

B Lymphocyte Induced Maturation Protein

Alternate Names

PRDM1, ZNFPR1A1

Entrez Gene IDs

639 (Human); 12142 (Mouse); 309871 (Rat)

Gene Symbol

PRDM1

UniProt

O75626

Additional BLIMP1/PRDM1 Products

Product Documents for Human BLIMP1/PRDM1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BLIMP1/PRDM1 Antibody

For research use only

Citations for Human BLIMP1/PRDM1 Antibody

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