Human BLIMP1/PRDM1 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human BLIMP1/PRDM1 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 646702
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human BLIMP1/PRDM1
    Lys667-Cys789
    Accession # O75626
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human BLIMP1/PRDM1 by Western Blot.

Western blot shows lysates of U266 human myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BLIMP1/PRDM1 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry
BLIMP1/PRDM1 in U266 Human Cell Line. BLIMP1/PRDM1 was detected in immersion fixed U266 human myeloma cell line using Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human BLIMP1/PRDM1 by Simple WesternTM. Simple Western lane view shows lysates of U266 human myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for BLIMP1/PRDM1 at approximately 114 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BLIMP1/PRDM1

Human BLIMP1 (B lymphocyte-induced maturation protein 1, also known as PRDM1) is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 is 89% aa identical to mouse BLIMP1.

  • Long Name:
    B Lymphocyte Induced Maturation Protein
  • Entrez Gene IDs:
    639 (Human); 12142 (Mouse); 309871 (Rat)
  • Alternate Names:
    Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP1; BLIMP-1; BLIMP1MGC118925; blmp-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI-BF1MGC118924; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1; PR-domain zinc finger protein 1; ZNFPR1A1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. IMiD immunomodulatory compounds block C/EBP{beta} translation through eIF4E down-regulation resulting in inhibition of MM.
    Authors: Li S, Pal R, Monaghan SA, Schafer P, Ouyang H, Mapara M, Galson DL, Lentzsch S
    Blood, 2011;117(19):5157-65.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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