Human BMI-1 Fluorescein-conjugated Antibody
Human BMI-1 Fluorescein-conjugated Antibody Summary
Asp96-Gly326
Accession # P35226
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of BMI‑1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human BMI-1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC33341F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BMI-1
BMI-1 (B cell-specific Moloney-MLV integration site #1) is a 45 kDa proto-oncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain within amino acids (aa) 17-56, a nuclear location sequence (NLS) at aa 81-95 and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 shares 99%, 97%, 99% and 99% aa sequence identity with bovine, mouse, feline and canine BMI-1, respectively.
Product Datasheets
Citation for Human BMI-1 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Expression of ABCG 2 and B mi‐1 in oral potentially malignant lesions and oral squamous cell carcinoma
Authors: Andrew J. Dalley, Luke P. Pitty, Aidan G. Major, Ahmad A. AbdulMajeed, Camile S. Farah
Cancer Medicine
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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10^6 MCF-7 cells were incubated with 10 ul of FITC-conjugated Mouse anti-Human BMI-1 antibody (red), or Mouse Isotype Control IgG (black). Buffer: 1% BSA in PBS.
